High-Fat Diet Augments the Effect of Alcohol on Skeletal Muscle Mitochondrial Dysfunction in Mice

Ahmed Ismaeel, Joseph A. Laudato, Emma Fletcher, Evlampia Papoutsi, Abigail Tice, Lara S. Hwa, Dimitrios Miserlis, Athanasios Z. Jamurtas, Jennifer Steiner, Panagiotis Koutakis

Research output: Contribution to journalArticlepeer-review

Abstract

Previous studies have shown that chronic heavy alcohol consumption and consumption of a high-fat (HF) diet can independently contribute to skeletal muscle oxidative stress and mitochondrial dysfunction, yet the concurrent effect of these risk factors remains unclear. We aimed to assess the effect of alcohol and different dietary compositions on mitochondrial activity and oxidative stress markers. Male and female mice were randomized to an alcohol (EtOH)-free HF diet, a HF + EtOH diet, or a low-Fat (LF) + EtOH diet for 6 weeks. At the end of the study, electron transport chain complex activity and expression as well as antioxidant activity and expression, were measured in skeletal muscles. Complex I and III activity were diminished in muscles of mice fed a HF + EtOH diet relative to the EtOH-free HF diet. Lipid peroxidation was elevated, and antioxidant activity was diminished, in muscles of mice fed a HF + EtOH diet as well. Consumption of a HF diet may exacerbate the negative effects of alcohol on skeletal muscle mitochondrial health and oxidative stress.

Original languageEnglish (US)
Article number1016
JournalNutrients
Volume14
Issue number5
DOIs
StatePublished - Mar 1 2022

Keywords

  • Alcohol
  • Aldehyde dehydrogenase 2
  • Mitochondrial function
  • Oxidative stress

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

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