TY - JOUR
T1 - Hexose monophosphate shunt metabolism in sheep
T2 - Comparison of fetal, newborn and adult erythrocytes
AU - Noble, N. A.
AU - Kuwashima, L. H.
AU - Davidson, W. D.
AU - Nathanielsz, P. W.
AU - Tanaka, K. R.
PY - 1981/12/1
Y1 - 1981/12/1
N2 - Glucose utilization through the hexose monophosphate shunt was measured in erythrocytes from fetal sheep, newborn lambs and adult pregnant sheep. Fetal erythrocytes demonstrate dramatically increased glucose oxidation in the presence of the oxidant new methylene blue. This response declines by birth and thereafter until adult sheep erythrocytes show no response. No stimulation was observed in adult sheep red cells under a number of conditions, although when adult sheep red cells were separated according to cell age, young cells were found to respond greatly to new methylene blue. When the rate of glucose oxidation after new methylene blue stimulation is correlated with in vitro glucose-6-phosphate dehydrogenase activity, a highly significant relationship is seen. This suggests that developmental changes in glucose oxidation by sheep red cells may be due to similar changes in glucose-6-phosphate dehydrogenase activity. The relationship also suggests that mature adult erythrocytes may be unable to respond to oxidants because of their low glucose-6-phosphate dehydrogenase activity.
AB - Glucose utilization through the hexose monophosphate shunt was measured in erythrocytes from fetal sheep, newborn lambs and adult pregnant sheep. Fetal erythrocytes demonstrate dramatically increased glucose oxidation in the presence of the oxidant new methylene blue. This response declines by birth and thereafter until adult sheep erythrocytes show no response. No stimulation was observed in adult sheep red cells under a number of conditions, although when adult sheep red cells were separated according to cell age, young cells were found to respond greatly to new methylene blue. When the rate of glucose oxidation after new methylene blue stimulation is correlated with in vitro glucose-6-phosphate dehydrogenase activity, a highly significant relationship is seen. This suggests that developmental changes in glucose oxidation by sheep red cells may be due to similar changes in glucose-6-phosphate dehydrogenase activity. The relationship also suggests that mature adult erythrocytes may be unable to respond to oxidants because of their low glucose-6-phosphate dehydrogenase activity.
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M3 - Article
C2 - 7347348
AN - SCOPUS:0019725531
VL - 3
SP - 333
EP - 341
JO - Journal of Developmental Physiology
JF - Journal of Developmental Physiology
SN - 0141-9846
IS - 6
ER -