Heterogeneity of the procapsid of bacteriophage T3

P. Serwer, R. H. Watson, S. J. Hayes

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Like other double-stranded DNA bacteriophages, bacteriophage T3 assembles a DNA-free procapsid that subsequently packages the bacteriophage DNA. By agarose gel electrophoresis, it has been found that the T3 procapsid has a negative electrophoretic mobility (μ) that progressively increases in magnitude by as much as 3% after assembly of the procapsid. This increase is (1) caused by an increase in the solid support-free μ (μ(o)) of the procapsid, not a decrease in its radius, and (2) not prevented by either genetically or chemically (use of proflavine) blocking DNA packaging. However, inhibition of the formation of high-energy compounds with a mixture of cyanide and fluoride ions does block the time-dependent increase in the magnitude of μ(o). This increase appears to be accompanied by addition of an undentified T3 protein to the procapsid.

Original languageEnglish (US)
Pages (from-to)232-237
Number of pages6
JournalJournal of virology
Volume55
Issue number1
DOIs
StatePublished - 1985

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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