Hepatoma-derived growth factor (HDGF) is dispensable for normal mouse development

Rainer Gallitzendoerfer, Mekky M. Abouzied, Dieter Hartmann, Radoslaw Dobrowolski, Volkmar Gieselmann, Sebastian Franken

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Hepatoma-derived growth factor (HDGF) is suggested to be involved in organ development and exhibits proliferative, angiogenic, and neurotrophic activity. The in vivo functions are, however, so far unknown. In this study, we generated HDGF-deficient mice, in which parts of the HDGF gene were replaced by a gene encoding green fluorescent protein (eGFP). HDGF-/- mice are viable with no apparent morphological abnormalities. Cultured HDGF-deficient dermal fibroblasts show unaltered proliferation rates and cell-cycle distributions. In contrast to previous studies, our data demonstrate that signal pathways involved in the response to extracellular HDGF do not depend on the presence of intracellular HDGF. Contrary to the reported role of HDGF as a modulator of apoptosis, similar apoptotic rates were found between wild-type and HDGF-deficient fibroblasts following tumor necrosis factor α (TNFα) -induced apoptosis or cellular stress. The lack of obvious biochemical and morphological phenotypes in HDGF-deficient mice demonstrates that in vivo HDGF is dispensable for normal development in mice.

Original languageEnglish (US)
Pages (from-to)1875-1885
Number of pages11
JournalDevelopmental Dynamics
Volume237
Issue number7
DOIs
StatePublished - Jul 1 2008

Keywords

  • Apoptosis
  • Cell proliferation
  • Development
  • HDGF

ASJC Scopus subject areas

  • Developmental Biology

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    Gallitzendoerfer, R., Abouzied, M. M., Hartmann, D., Dobrowolski, R., Gieselmann, V., & Franken, S. (2008). Hepatoma-derived growth factor (HDGF) is dispensable for normal mouse development. Developmental Dynamics, 237(7), 1875-1885. https://doi.org/10.1002/dvdy.21589