Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) interacts with PELP1 and activates MAPK

Suresh K. Rayala, Petra Den Hollander, Seetharaman Balasenthil, Poonam R. Molli, Andrew J. Bean, Ratna K Vadlamudi, Rui An Wang, Rakesh Kumar

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

PELP1 (proline-, glutamic acid-, and leucine-rich protein-1) (also known as the modulator of nongenomic activity of estrogen receptor) plays a role in genomic functions of the estrogen receptor via histone interactions and in nongenomic functions via its influence on the MAPK-Src pathway. However, recent studies have shown that differential compartmentalization of PELP1 could play a crucial role in modulating the status of nongenomic signaling by using molecular mechanisms that remain poorly understood. Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) is an early endosomal protein that plays a role in regulating the trafficking of growth factor-receptor complexes through early endosomes. By using a yeast two-hybrid screen, we identified HRS as a novel PELP1-binding protein providing evidence of a physiologic interaction between HRS and PELP1. The noted HRS-PELP1 interaction was accompanied by inhibition of the basal coactivator function of PELP1 upon estrogen receptor transactivation. HRS was found to sequester PELP1 in the cytoplasm, leading to the activation of MAPK in amanner that is dependent on the epidermal growth factor receptor but independent of the estrogen receptor, Shc, and Src. In addition, stimulation of MAPK and the subsequent activation of its downstream effector pathway, Elk-1, by HRS or PELP1 were found to depend on the presence of endogenous PELP1 or HRS. Furthermore, HRS was overexpressed and correlated well with the cytoplasmic PELP1, increased MAPK, and EGFR status in breast tumors. These findings highlight a novel role of HRS in up-regulating MAPK, presumably involving interaction with PELP1.

Original languageEnglish (US)
Pages (from-to)4395-4403
Number of pages9
JournalJournal of Biological Chemistry
Volume281
Issue number7
DOIs
StatePublished - Feb 17 2006
Externally publishedYes

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Estrogen Receptors
human PELP1 protein
hepatocyte growth factor-regulated tyrosine kinase substrate
Chemical activation
Growth Factor Receptors
Endosomes
Epidermal Growth Factor Receptor
Protein Binding
Histones
Yeast
Modulators
Transcriptional Activation
Tumors
Carrier Proteins
Cytoplasm
Yeasts
Breast Neoplasms
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) interacts with PELP1 and activates MAPK. / Rayala, Suresh K.; Den Hollander, Petra; Balasenthil, Seetharaman; Molli, Poonam R.; Bean, Andrew J.; Vadlamudi, Ratna K; Wang, Rui An; Kumar, Rakesh.

In: Journal of Biological Chemistry, Vol. 281, No. 7, 17.02.2006, p. 4395-4403.

Research output: Contribution to journalArticle

Rayala, SK, Den Hollander, P, Balasenthil, S, Molli, PR, Bean, AJ, Vadlamudi, RK, Wang, RA & Kumar, R 2006, 'Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) interacts with PELP1 and activates MAPK', Journal of Biological Chemistry, vol. 281, no. 7, pp. 4395-4403. https://doi.org/10.1074/jbc.M510368200
Rayala, Suresh K. ; Den Hollander, Petra ; Balasenthil, Seetharaman ; Molli, Poonam R. ; Bean, Andrew J. ; Vadlamudi, Ratna K ; Wang, Rui An ; Kumar, Rakesh. / Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) interacts with PELP1 and activates MAPK. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 7. pp. 4395-4403.
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AU - Den Hollander, Petra

AU - Balasenthil, Seetharaman

AU - Molli, Poonam R.

AU - Bean, Andrew J.

AU - Vadlamudi, Ratna K

AU - Wang, Rui An

AU - Kumar, Rakesh

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