Hepatitis C virus F protein is a short-lived protein associated with the endoplasmic reticulum

Zhenming Xu, Jinah Choi, Wen Lu, Jing hsiung Ou

Research output: Contribution to journalArticlepeer-review

62 Scopus citations


Hepatitis C virus (HCV) F protein is a newly discovered HCV gene product that is expressed by translational ribosomal frameshift. Little is known about the biological properties of this protein. By performing pulse-chase labeling experiments, we demonstrate here that the F protein is a labile protein with a half-life of < 10 min in Huh7 hepatoma cells and in vitro. The half-life of the F protein could be substantially increased by proteasome inhibitors, suggesting that the rapid degradation of the F protein is mediated by the proteasome pathway. Further immunofluorescence staining and subcellular fractionation experiments indicate that the F protein is primarily associated with the endoplasmic reticulum. This subcellular localization is similar to those of HCV core and NSSA proteins, raising the possibility that the F protein may participate in HCV morphogenesis or replication.

Original languageEnglish (US)
Pages (from-to)1578-1583
Number of pages6
JournalJournal of virology
Issue number2
StatePublished - Jan 2003
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology


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