Heparin-binding EGF-like growth factor protects intestinal stem cells from injury in a rat model of necrotizing enterocolitis

Chun-liang Chen, Xiaoyi Yu, Iyore O A James, Hong Yi Zhang, Jingyuan Yang, Andrei Radulescu, Yu Zhou, Gail E. Besner

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Necrotizing enterocolitis (NEC) is an often catastrophic disease that typically affects premature newborns. Although the exact etiology of NEC is uncertain, the disease is associated with formula feeding, bacterial colonization of the gut, hypoxia and hypoperfusion. In light of the pathogenesis of NEC, the integrity and function of the intestinal mucosa has a major defensive role against the initiation of NEC. Various forms of intestinal injury, including NEC, injure the intestinal epithelial cell (IEC) lineages, including the intestinal stem cells (ISCs), thereby disrupting the normal homeostasis needed to maintain gut barrier function. In the current study, we examined the effects of heparin-binding EGF-like growth factor (HB-EGF) administration on enterocytes, goblet cells, neuroendocrine cells and ISCs in a newborn rat model of experimental NEC. We also examined the cytoprotective effects of HB-EGF on ISCs in in vitro cell cultures and in ex vivo crypt-villous organoid cultures. We found that HB-EGF protects all IEC lineages, including ISCs, from injury. We further found that HB-EGF protects isolated ISCs from hypoxic injury in vitro, and promotes ISC activation and survival, and the expansion of crypt transit-amplifying cells, in ex vivo crypt-villous organoid cultures. The protective effects of HB-EGF were dependent on EGF receptor activation, and were mediated via the MEK1/2 and PI3K signaling pathways. These results show that the intestinal cytoprotective effects of HB-EGF are mediated, at least in part, through its ability to protect ISCs from injury.

Original languageEnglish (US)
Pages (from-to)331-344
Number of pages14
JournalLaboratory Investigation
Volume92
Issue number3
DOIs
StatePublished - Mar 2012
Externally publishedYes

Fingerprint

Necrotizing Enterocolitis
Stem Cells
Wounds and Injuries
Organoids
Cell Lineage
Epithelial Cells
Neuroendocrine Cells
Goblet Cells
Enterocytes
Intestinal Mucosa
Heparin-binding EGF-like Growth Factor
Phosphatidylinositol 3-Kinases
Epidermal Growth Factor Receptor
Cell Survival
Homeostasis
Theoretical Models
Cell Culture Techniques

Keywords

  • epithelial cells
  • HB-EGF
  • intestine
  • LGR5
  • prominin-1
  • stem cells

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Molecular Biology

Cite this

Heparin-binding EGF-like growth factor protects intestinal stem cells from injury in a rat model of necrotizing enterocolitis. / Chen, Chun-liang; Yu, Xiaoyi; James, Iyore O A; Zhang, Hong Yi; Yang, Jingyuan; Radulescu, Andrei; Zhou, Yu; Besner, Gail E.

In: Laboratory Investigation, Vol. 92, No. 3, 03.2012, p. 331-344.

Research output: Contribution to journalArticle

Chen, Chun-liang ; Yu, Xiaoyi ; James, Iyore O A ; Zhang, Hong Yi ; Yang, Jingyuan ; Radulescu, Andrei ; Zhou, Yu ; Besner, Gail E. / Heparin-binding EGF-like growth factor protects intestinal stem cells from injury in a rat model of necrotizing enterocolitis. In: Laboratory Investigation. 2012 ; Vol. 92, No. 3. pp. 331-344.
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