Hemolytic and hemoxidative activities in Mycoplasma penetrans

Thirumalai R Kannan, J. B. Baseman

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Mycoplasma penetrans is a newly isolated Mollicute from the urine of patients infected with human immunodeficiency virus that demonstrates the capacity to adhere to and invade human cells. A previous report, based on assays with mouse red blood cells (RBCs), indicated that M. penetrans lacked hemolytic activity. In our studies, we incubated different isolates of M. penetrans with various RBC species and observed hemolytic zones surrounding individual mycoplasma colonies. All M. penetrans strains displayed hemolysis after 2 to 3 days of incubation. Hemolytic activity diffused from single colonies, eventually causing complete lysis. Hemolysis was most pronounced with sheep RBCs, followed by horse, chicken, and human cells. Furthermore, hemolytic activity was demonstrable in both intact mycoplasma cell preparations and spent culture supernatant. However, unlike intact mycoplasmas, the hemolytic activity in the supernatant was dependent on the reducing agent, cysteine. In addition to hemolysis, a brown precipitate was closely associated with mycoplasma colonies, suggesting oxidation of hemoglobin. Absorption spectra indicated that hemoglobin was oxidized to methemoglobin, and the addition of catalase demonstrated H2O2-mediated hemoxidation. Other experiments suggested that hemoxidation enhanced total hemolysis, providing the first evidence of both hemolytic and hemoxidative activities in M. penetrans.

Original languageEnglish (US)
Pages (from-to)6419-6422
Number of pages4
JournalInfection and Immunity
Volume68
Issue number11
DOIs
StatePublished - 2000

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Mycoplasma penetrans
Mycoplasma
Hemolysis
Erythrocytes
Hemoglobins
Tenericutes
Methemoglobin
Reducing Agents
Catalase
Horses
Cysteine
Chickens
Sheep
HIV
Urine

ASJC Scopus subject areas

  • Immunology

Cite this

Hemolytic and hemoxidative activities in Mycoplasma penetrans. / Kannan, Thirumalai R; Baseman, J. B.

In: Infection and Immunity, Vol. 68, No. 11, 2000, p. 6419-6422.

Research output: Contribution to journalArticle

Kannan, Thirumalai R ; Baseman, J. B. / Hemolytic and hemoxidative activities in Mycoplasma penetrans. In: Infection and Immunity. 2000 ; Vol. 68, No. 11. pp. 6419-6422.
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