Hemolytic activity in the periodontopathogen Porphyromonas gingivalis: Kinetics of enzyme release and localization

Lianrui Chu, T. E. Bramanti, J. L. Ebersole, S. C. Holt

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Abstract

Porphyromonas gingivalis W50, W83, A7A1-28, and ATCC 33277 were investigated for their abilities to lyse sheep, human, and rabbit erythrocytes. All of the P. gingivalis strains studied produced an active hemolytic activity during growth, with maximum activity occurring in late-exponential-early-stationary growth phase. The enzyme was cell bound and associated with the outer membrane. Fractionation of P. gingivalis W50 localized the putative hemolysin almost exclusively in the outer membrane fraction, with significant hemolytic activity concentrated in the outer membrane vesicles. Ca2+ and Mg2+ ions significantly increased the expression of hemolytic activity. Hemolytic activity was inhibited by proteinase K, trypsin, the proteinase inhibitors Na-P-tosyl-L-lysine chloromethyl ketone and benzamidine, the metabolic inhibitor M-chlorophenyl-hydrazone, and iodoacetate. KCN and sodium azide (NaN3) only partially inhibited P. gingivalis hemolytic activity, while antiserum to whole cells of each of the P. gingivalis strains had a significant inhibitory effect on hemolytic activity. The P. gingivalis W50 hemolysin was inhibited by cysteine, dithiothreitol, and glutathione at concentrations of at least 10 mM; at low concentrations (i.e., 2 mM), dithiothreitol did not completely inhibit hemolytic activity. Heating to temperatures above 55°C resulted in an almost complete inhibition of hemolytic activity. The effect of heme limitation (i.e., iron) on hemolysin production indicated that either limitation or starvation for heme resulted in significantly increased hemolysin production compared with that of P. gingivalis grown in the presence of excess heme.

Original languageEnglish (US)
Pages (from-to)1932-1940
Number of pages9
JournalInfection and Immunity
Volume59
Issue number6
StatePublished - 1991

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Porphyromonas gingivalis
Hemolysin Proteins
Enzymes
Heme
Sodium Azide
Dithiothreitol
Membranes
Iodoacetates
Hydrazones
Endopeptidase K
Trypsin Inhibitors
Growth
Starvation
Heating
Lysine
Glutathione
Cysteine
Immune Sera
Sheep
Peptide Hydrolases

ASJC Scopus subject areas

  • Immunology

Cite this

Hemolytic activity in the periodontopathogen Porphyromonas gingivalis : Kinetics of enzyme release and localization. / Chu, Lianrui; Bramanti, T. E.; Ebersole, J. L.; Holt, S. C.

In: Infection and Immunity, Vol. 59, No. 6, 1991, p. 1932-1940.

Research output: Contribution to journalArticle

Chu, Lianrui ; Bramanti, T. E. ; Ebersole, J. L. ; Holt, S. C. / Hemolytic activity in the periodontopathogen Porphyromonas gingivalis : Kinetics of enzyme release and localization. In: Infection and Immunity. 1991 ; Vol. 59, No. 6. pp. 1932-1940.
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abstract = "Porphyromonas gingivalis W50, W83, A7A1-28, and ATCC 33277 were investigated for their abilities to lyse sheep, human, and rabbit erythrocytes. All of the P. gingivalis strains studied produced an active hemolytic activity during growth, with maximum activity occurring in late-exponential-early-stationary growth phase. The enzyme was cell bound and associated with the outer membrane. Fractionation of P. gingivalis W50 localized the putative hemolysin almost exclusively in the outer membrane fraction, with significant hemolytic activity concentrated in the outer membrane vesicles. Ca2+ and Mg2+ ions significantly increased the expression of hemolytic activity. Hemolytic activity was inhibited by proteinase K, trypsin, the proteinase inhibitors Na-P-tosyl-L-lysine chloromethyl ketone and benzamidine, the metabolic inhibitor M-chlorophenyl-hydrazone, and iodoacetate. KCN and sodium azide (NaN3) only partially inhibited P. gingivalis hemolytic activity, while antiserum to whole cells of each of the P. gingivalis strains had a significant inhibitory effect on hemolytic activity. The P. gingivalis W50 hemolysin was inhibited by cysteine, dithiothreitol, and glutathione at concentrations of at least 10 mM; at low concentrations (i.e., 2 mM), dithiothreitol did not completely inhibit hemolytic activity. Heating to temperatures above 55°C resulted in an almost complete inhibition of hemolytic activity. The effect of heme limitation (i.e., iron) on hemolysin production indicated that either limitation or starvation for heme resulted in significantly increased hemolysin production compared with that of P. gingivalis grown in the presence of excess heme.",
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