Hemoglobin Ypsilanti: A high-oxygen-affinity hemoglobin demonstrated by two automated high-pressure liquid chromatography systems

Daniel D. Mais, Laurence A. Boxer, Ronald D. Gulbranson, David F. Keren

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Hemoglobin (Hb) Ypsilanti is a rare high-oxygen-affinity hemoglobin. Like other high-oxygen-affinity hemoglobins, Hb Ypsilanti manifests as erythrocytosis. Because the migration of many high-oxygen-affinity variants on alkaline and acid gels does not differ from that of HbA, oxygen-hemoglobin dissociation studies are often used to document their presence. Hb Ypsilanti is a notable exception because its electrophoresis pattern on alkaline gel is highly characteristic, exemplifying the phenomenon of hybrid formation in variant hemoglobins. In the past few years, several laboratories have begun to use high-pressure liquid chromatography (HPLC) as a screen for hemoglobinopathies. We demonstrate the elution profile of Hb Ypsilanti on the 2 most widely used HPLC methods.

Original languageEnglish (US)
Pages (from-to)850-853
Number of pages4
JournalAmerican journal of clinical pathology
Volume128
Issue number5
DOIs
StatePublished - Nov 1 2007
Externally publishedYes

Keywords

  • Electrophoresis
  • Erythrocytosis
  • Hemoglobin Ypsilanti
  • High-oxygen-affinity hemoglobin
  • High-pressure liquid chromatography

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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