Hemoglobin transition in erythrocytes of developing chick. Studies with cell-free protein-synthesizing systems

A. Burl Henderson, John C. Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cell-free hemoglobin-synthesizing systems from erythrocytes of 4- and 17-day chick embryos have been developed. These systems have been used to investigate possible structural and functional differences in factors involved in protein synthesis obtained from these different developmental stages. Each cell-free system consists of three major cellular fractions i.e., the S-100 supernatant, the salt-washed ribosomes, and the 0.5 m KCl ribosomal wash. When the ribosomal wash fraction from one developmental stage is included in a cell-free system containing ribosomes and S-100 supernatant from the other developmental stage, a drastic reduction in the kinetics of [3H]leucine incorporation into globin products is observed, when compared to the homologous control cell-free systems. A similar depression of the kinetics of incorporation is observed when the mixed component is either the S-100 supernatant or the ribosomes. Control rates of incorporation can be reestablished when the corresponding homologous component is added back to the incubation mixture. The predominant types of hemoglobins produced in the salt-wash heterologous systems are those hemoglobins characteristic of the developmental stage of the salt wash. This seems to imply that the ribosomal salt-wash fraction may possess developmental stage specificity for the globins.

Original languageEnglish (US)
Pages (from-to)637-646
Number of pages10
JournalArchives of Biochemistry and Biophysics
Volume174
Issue number2
DOIs
StatePublished - 1976

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Cell-Free System
Hemoglobins
Salts
Erythrocytes
Ribosomes
Globins
Proteins
Kinetics
Chick Embryo
Leucine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Hemoglobin transition in erythrocytes of developing chick. Studies with cell-free protein-synthesizing systems. / Henderson, A. Burl; Lee, John C.

In: Archives of Biochemistry and Biophysics, Vol. 174, No. 2, 1976, p. 637-646.

Research output: Contribution to journalArticle

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