Heme oxygenase expression in human placenta and placental bed

Reduced expression of placenta endothelial HO-2 in preeclampsia and fetal growth restriction

Andrew Barber, Stephen Courtenay Robson, Leslie Myatt, Judith Nicola Bulmer, Fiona Lyall

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

In this study we tested the hypothesis that expression of heme oxygenases HO-1 and HO-2, which are responsible for the production of carbon monoxide, are reduced in the placenta and placental bed of pregnancies complicated by preeclampsia (PE) and fetal growth restriction (FGR) compared with control third-trimester pregnancies. Placental protein expression was determined by Western blotting (n=10 in each group) and immunohistochemistry (controls n=18, PE n=19, FGR n=10). Extravillous trophoblast expression was determined by immunohistochemistry of placental bed biopsy samples (controls n=17, PE n=19, FGR n=10). Western blot analysis of placental homogenates showed no overall differences in HO-2 among groups. However, immunohistochemical analysis showed a reduction in HO-2 expression in endothelial cells in both abnormal groups (PE P<0.01; FGR P<0.0005 vs. control group) but no differences in villous trophoblast staining. HO-1 was undetectable by Western blotting in control and abnormal pregnancies and immunoreactivity was very low, suggesting that there is little HO-1 in the placenta. Within the placental bed, HO-2 but not HO-1 was detected on all populations of extravillous trophoblast, but expression of HO-2 or HO-1 did not change in PE or FGR. The reduced expression of HO-2 on endothelial cells in PE and FGR may be responsible for reduced placental blood flow in these conditions. The data do not show changes in HO in the placental bed in PE or FGR. - Barber, A., Robson, S. C., Myatt, L., Bulmer, J. N., Lyall, F. Heme oxygenase expression in human placenta and placental bed: reduced expression of placenta endothelial HO-2 in preeclampsia and fetal growth restriction.

Original languageEnglish (US)
Pages (from-to)1158-1168
Number of pages11
JournalFASEB Journal
Volume15
Issue number7
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

pre-eclampsia
Heme Oxygenase (Decyclizing)
fetal development
heme
oxygenases
Fetal Development
Pre-Eclampsia
placenta
Placenta
trophoblast
Trophoblasts
Western blotting
Western Blotting
Endothelial cells
pregnancy
endothelial cells
heme oxygenase (biliverdin-producing)
immunohistochemistry
Endothelial Cells
Immunohistochemistry

Keywords

  • Carbon monoxide
  • HO-1
  • HO-2
  • Pregnancy
  • Trophoblast

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Heme oxygenase expression in human placenta and placental bed : Reduced expression of placenta endothelial HO-2 in preeclampsia and fetal growth restriction. / Barber, Andrew; Robson, Stephen Courtenay; Myatt, Leslie; Bulmer, Judith Nicola; Lyall, Fiona.

In: FASEB Journal, Vol. 15, No. 7, 2001, p. 1158-1168.

Research output: Contribution to journalArticle

Barber, Andrew ; Robson, Stephen Courtenay ; Myatt, Leslie ; Bulmer, Judith Nicola ; Lyall, Fiona. / Heme oxygenase expression in human placenta and placental bed : Reduced expression of placenta endothelial HO-2 in preeclampsia and fetal growth restriction. In: FASEB Journal. 2001 ; Vol. 15, No. 7. pp. 1158-1168.
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AB - In this study we tested the hypothesis that expression of heme oxygenases HO-1 and HO-2, which are responsible for the production of carbon monoxide, are reduced in the placenta and placental bed of pregnancies complicated by preeclampsia (PE) and fetal growth restriction (FGR) compared with control third-trimester pregnancies. Placental protein expression was determined by Western blotting (n=10 in each group) and immunohistochemistry (controls n=18, PE n=19, FGR n=10). Extravillous trophoblast expression was determined by immunohistochemistry of placental bed biopsy samples (controls n=17, PE n=19, FGR n=10). Western blot analysis of placental homogenates showed no overall differences in HO-2 among groups. However, immunohistochemical analysis showed a reduction in HO-2 expression in endothelial cells in both abnormal groups (PE P<0.01; FGR P<0.0005 vs. control group) but no differences in villous trophoblast staining. HO-1 was undetectable by Western blotting in control and abnormal pregnancies and immunoreactivity was very low, suggesting that there is little HO-1 in the placenta. Within the placental bed, HO-2 but not HO-1 was detected on all populations of extravillous trophoblast, but expression of HO-2 or HO-1 did not change in PE or FGR. The reduced expression of HO-2 on endothelial cells in PE and FGR may be responsible for reduced placental blood flow in these conditions. The data do not show changes in HO in the placental bed in PE or FGR. - Barber, A., Robson, S. C., Myatt, L., Bulmer, J. N., Lyall, F. Heme oxygenase expression in human placenta and placental bed: reduced expression of placenta endothelial HO-2 in preeclampsia and fetal growth restriction.

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