Hematopoietic lineage- and stage-restricted expression of the ETS oncogene family member PU.1

Robert Hromas; Attilio Orazi; Richard S. Neiman; Richard Maki; Chuck Van Beveran; Jason Moore; Michael Klemsz

doi:10.1182/blood.v82.10.2998.2998

Hematopoietic lineage- and stage-restricted expression of the ETS oncogene family member PU.1

Robert Hromas, Attilio Orazi, Richard S. Neiman, Richard Maki, Chuck Van Beveran, Jason Moore, Michael Klemsz

Research output: Contribution to journal › Article › peer-review

203 Scopus citations

Abstract

The ETS oncogene family member PU.1 is a transcriptional activator that is dysregulated by Friend erythroleukemia virus insertion. Northern analysis found that PU.1 is highly expressed in cells of myeloid and B-lymphoid origin, but not expressed at all in a number of nonhematopoietic tissues. Interferon-γ and retinoic acid downregulated PU.1 expression in marrow macrophages. In situ immunohistochemistry found that PU.1 is expressed only in early granulocytic and erythroid cells and megakaryocytes, but not in mature erythroid cells, mature granulocytes, endothelial cells, or osteocytes. Thus, its expression pattern makes PU.1 a candidate for a genetic determinant of lineage commitment and stage progression in blood cell development. It also lends insight into how PU.1 might play a role in Friend virus erythroleukemia.

Original language	English (US)
Pages (from-to)	2998-3004
Number of pages	7
Journal	Blood
Volume	82
Issue number	10
DOIs	https://doi.org/10.1182/blood.v82.10.2998.2998
State	Published - Nov 15 1993
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Immunology
Hematology
Cell Biology

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10.1182/blood.v82.10.2998.2998

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title = "Hematopoietic lineage- and stage-restricted expression of the ETS oncogene family member PU.1",

abstract = "The ETS oncogene family member PU.1 is a transcriptional activator that is dysregulated by Friend erythroleukemia virus insertion. Northern analysis found that PU.1 is highly expressed in cells of myeloid and B-lymphoid origin, but not expressed at all in a number of nonhematopoietic tissues. Interferon-γ and retinoic acid downregulated PU.1 expression in marrow macrophages. In situ immunohistochemistry found that PU.1 is expressed only in early granulocytic and erythroid cells and megakaryocytes, but not in mature erythroid cells, mature granulocytes, endothelial cells, or osteocytes. Thus, its expression pattern makes PU.1 a candidate for a genetic determinant of lineage commitment and stage progression in blood cell development. It also lends insight into how PU.1 might play a role in Friend virus erythroleukemia.",

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AU - Hromas, Robert

AU - Orazi, Attilio

AU - Neiman, Richard S.

AU - Maki, Richard

AU - Van Beveran, Chuck

AU - Moore, Jason

AU - Klemsz, Michael

PY - 1993/11/15

Y1 - 1993/11/15

N2 - The ETS oncogene family member PU.1 is a transcriptional activator that is dysregulated by Friend erythroleukemia virus insertion. Northern analysis found that PU.1 is highly expressed in cells of myeloid and B-lymphoid origin, but not expressed at all in a number of nonhematopoietic tissues. Interferon-γ and retinoic acid downregulated PU.1 expression in marrow macrophages. In situ immunohistochemistry found that PU.1 is expressed only in early granulocytic and erythroid cells and megakaryocytes, but not in mature erythroid cells, mature granulocytes, endothelial cells, or osteocytes. Thus, its expression pattern makes PU.1 a candidate for a genetic determinant of lineage commitment and stage progression in blood cell development. It also lends insight into how PU.1 might play a role in Friend virus erythroleukemia.

AB - The ETS oncogene family member PU.1 is a transcriptional activator that is dysregulated by Friend erythroleukemia virus insertion. Northern analysis found that PU.1 is highly expressed in cells of myeloid and B-lymphoid origin, but not expressed at all in a number of nonhematopoietic tissues. Interferon-γ and retinoic acid downregulated PU.1 expression in marrow macrophages. In situ immunohistochemistry found that PU.1 is expressed only in early granulocytic and erythroid cells and megakaryocytes, but not in mature erythroid cells, mature granulocytes, endothelial cells, or osteocytes. Thus, its expression pattern makes PU.1 a candidate for a genetic determinant of lineage commitment and stage progression in blood cell development. It also lends insight into how PU.1 might play a role in Friend virus erythroleukemia.

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Hematopoietic lineage- and stage-restricted expression of the ETS oncogene family member PU.1

Abstract

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