Helminth induced suppression of macrophage activation is correlated with inhibition of calcium channel activity

Arun Chauhan, Yuyang Sun, Biswaranjan Pani, Fredice Quenumzangbe, Jyotika Sharma, Brij B Singh, Bibhuti B. Mishra

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Helminth parasites cause persistent infections in humans and yet many infected individuals are asymptomatic. Neurocysticercosis (NCC), a disease of the central nervous system (CNS) caused by the cestode Taenia solium, has a long asymptomatic phase correlated with an absence of brain inflammation. However, the mechanisms of immune suppression remain poorly understood. Here we report that murine NCC displays a lack of cell surface maturation markers in infiltrating myeloid cells. Furthermore, soluble parasite ligands (PL) failed to induce maturation of macrophages, and inhibited TLRinduced inflammatory cytokine production. Importantly, PL treatment abolished both LPS and thapsigargin-induced store operated Ca2+ entry (SOCE). Moreover, electrophysiological recordings demonstrated PL-mediated inhibition of LPS or Tginduced currents that were TRPC1-dependent. Concomitantly STIM1-TRPC1 complex was also impaired that was essential for SOCE and sustained Ca2+ entry. Likewise loss of SOCE due to PL further inhibited NFkB activation. Overall, our results indicate that the negative regulation of agonist induced Ca2+ signaling pathway by parasite ligands may be a novel immune suppressive mechanism to block the initiation of the inflammatory response associated with helminth infections.

Original languageEnglish (US)
Article numbere101023
JournalPLoS One
Volume9
Issue number7
DOIs
StatePublished - Jul 11 2014
Externally publishedYes

Fingerprint

macrophage activation
calcium channels
Macrophage Activation
Macrophages
Helminths
Calcium Channels
helminths
Parasites
Chemical activation
parasites
Ligands
calcium
neurocysticercosis
Neurocysticercosis
Taenia solium
helminthiasis
central nervous system diseases
Cestoda
Thapsigargin
Central Nervous System Diseases

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Helminth induced suppression of macrophage activation is correlated with inhibition of calcium channel activity. / Chauhan, Arun; Sun, Yuyang; Pani, Biswaranjan; Quenumzangbe, Fredice; Sharma, Jyotika; Singh, Brij B; Mishra, Bibhuti B.

In: PLoS One, Vol. 9, No. 7, e101023, 11.07.2014.

Research output: Contribution to journalArticle

Chauhan, Arun ; Sun, Yuyang ; Pani, Biswaranjan ; Quenumzangbe, Fredice ; Sharma, Jyotika ; Singh, Brij B ; Mishra, Bibhuti B. / Helminth induced suppression of macrophage activation is correlated with inhibition of calcium channel activity. In: PLoS One. 2014 ; Vol. 9, No. 7.
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