Handmade cloned transgenic piglets expressing the nematode fat-1 gene

Peng Zhang, Yidi Zhang, Hongwei Dou, Jingdong Yin, Yu Chen, Xinzhi Pang, Gabor Vajta, Lars Bolund, Yutao Du, Runlin Z. Ma

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Production of transgenic animals via somatic cell nuclear transfer (SCNT) has been adapted worldwide, but this application is somewhat limited by its relatively low efficiency. In this study, we used handmade cloning (HMC) established previously to produce transgenic pigs that express the functional nematode fat-1 gene. Codon-optimized mfat-1 was inserted into eukaryotic expression vectors, which were transferred into primary swine donor cells. Reverse transcriptase PCR (RT-PCR), gas chromatography, and chromosome analyses were performed to select donor clones capable of converting n-6 into n-3 fatty acids. Blastocysts derived from the clones that lowered the n-6/n-3 ratio to approximately 1:1 were transferred surgically into the uteri of recipients for transgenic piglets. By HMC, 37% (n=558) of reconstructed embryos developed to the blastocyst stage after 7 days of culture in vitro, with an average cell number of 81±36 (n=14). Three recipients became pregnant after 408 day-6 blastocysts were transferred into four naturally cycling females, and a total of 14 live offspring were produced. The nematode mfat-1 effectively lowered the n-6/n-3 ratio in muscle and major organs of the transgenic pig. Our results will help to establish a reliable procedure and an efficient option in the production of transgenic animals.

Original languageEnglish (US)
Pages (from-to)258-266
Number of pages9
JournalCellular Reprogramming
Volume14
Issue number3
DOIs
StatePublished - Jun 1 2012
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Developmental Biology
  • Cell Biology

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    Zhang, P., Zhang, Y., Dou, H., Yin, J., Chen, Y., Pang, X., Vajta, G., Bolund, L., Du, Y., & Ma, R. Z. (2012). Handmade cloned transgenic piglets expressing the nematode fat-1 gene. Cellular Reprogramming, 14(3), 258-266. https://doi.org/10.1089/cell.2011.0073