Halothane-induced changes in acetylcholine receptor channel kinetics are attenuated by cholesterol

James Lechleiter, Michael Wells, Raphael Gruener

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

The single-channel recording technique was used to investigate the role of membrane lipids in the action of general anesthetics on ion channels. We examined the effects of halothane on acetylcholine receptor channels in Xenopus laevis myocytes in which the plasma membrane cholesterol level had been changed by pretreatment with cholesterol-rich or cholesterol-free liposomes. We found that the alteration in acetylcholine receptor channel kinetics, elicited in the presence of clinically-relevant concentrations of halothane, is attenuated when membrane cholesterol is increased and enhanced when membrane cholesterol concentration is decreased. These findings support the idea that general anesthetics interact with synaptic receptor channels indirectly through the lipid domains in which these synaptic proteins are embedded.

Original languageEnglish (US)
Pages (from-to)640-645
Number of pages6
JournalBBA - Biomembranes
Volume856
Issue number3
DOIs
StatePublished - Apr 25 1986
Externally publishedYes

Keywords

  • (X. laevis myocyte)
  • Acetylcholine receptor channel
  • Anesthetic-membrane interaction
  • Halothane
  • Patch clamp

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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