Hairy cell leukaemia: effects of phorbol ester on cell morphology and adherence and comparison with other B‐cell neoplasias

Aaron Polliack, Dorit Gurfel, Reuven Or, Rachel Leizerowitz, Yair Gazitt

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

This report documents phorbol ester‐induced changes in cell morphology in 9 patients with hairy cell leukaemia (HCL) and contrasts them with those obtained in cells from 20 patients with B‐type chronic lymphocytic leukaemia (CLL) and non‐Hodgkin's lymphoma in leukaemic phase (NHL). The phorbol ester caused hairy cells to adhere strongly and produce thin elongated cytoplasmic extensions. Other cells showed marked surface ruffling, and contained increased numbers of phagolysosomes, although cells still retained ribosomal lamellar complexes. This in vitro growth pattern differed from that observed in B‐CLL and NHL cells, which readily detached in clumps with minimal adherence. CLL and NHL‐cells did not show macrophage features but developed plasmacytoid and hairy cell‐like features. It is suggested that these different growth patterns may aid in distinguishing HCL from other B‐cell neoplasias.

Original languageEnglish (US)
Pages (from-to)358-366
Number of pages9
JournalScandinavian Journal of Haematology
Volume36
Issue number4
DOIs
StatePublished - Apr 1986
Externally publishedYes

Keywords

  • adherence
  • CLL
  • hairy cell leukaernia
  • morphology
  • phorbol esters
  • TPA

ASJC Scopus subject areas

  • Hematology

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