H2O2 induced lipid peroxidation in rat brain homogenates is greatly reduced by melatonin

Ewa Sewerynek; Burkhard Poeggeler; Daniela Melchiorri; Russel J. Reiter

doi:10.1016/0304-3940(95)11803-5

H2O2 induced lipid peroxidation in rat brain homogenates is greatly reduced by melatonin

Ewa Sewerynek, Burkhard Poeggeler, Daniela Melchiorri, Russel J. Reiter

Department of Cell Systems & Anatomy

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

Melatonin protection against H₂O₂-induced lipid peroxidation in brain homogenates was measured in vitro. The level of malonaldehyde (MDA) plus 4-hydroxyalkenals (4-HDA) was assayed in brain homogenates as an index of induced membrane oxidative damage. Brain homogenates were co-incubated with H₂O₂ alone or in combination with melatonin. Brain MDA + 4-HDA increased after H202 (0.1-5 mM) with the effect being both concentration- and time-dependent. Melatonin (0.1-5 mM) protected against H₂O₂-induced lipid peroxidation of brain homogenates in a concentration-dependent manner.

Original language	English (US)
Pages (from-to)	203-205
Number of pages	3
Journal	Neuroscience Letters
Volume	195
Issue number	3
DOIs	https://doi.org/10.1016/0304-3940(95)11803-5
State	Published - Aug 11 1995

Keywords

Brain
HO
Hydroxyl radical
Lipid peroxidation
Melatonin

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/0304-3940(95)11803-5

Cite this

@article{0742386f8fab42caa563ce91eaad6720,

title = "H2O2 induced lipid peroxidation in rat brain homogenates is greatly reduced by melatonin",

abstract = "Melatonin protection against H2O2-induced lipid peroxidation in brain homogenates was measured in vitro. The level of malonaldehyde (MDA) plus 4-hydroxyalkenals (4-HDA) was assayed in brain homogenates as an index of induced membrane oxidative damage. Brain homogenates were co-incubated with H2O2 alone or in combination with melatonin. Brain MDA + 4-HDA increased after H202 (0.1-5 mM) with the effect being both concentration- and time-dependent. Melatonin (0.1-5 mM) protected against H2O2-induced lipid peroxidation of brain homogenates in a concentration-dependent manner.",

keywords = "Brain, HO, Hydroxyl radical, Lipid peroxidation, Melatonin",

author = "Ewa Sewerynek and Burkhard Poeggeler and Daniela Melchiorri and Reiter, {Russel J.}",

note = "Funding Information: E.S. was supported by a Fogarty International Fellowship, no. 1 FO5 TWO 5013-01 ICP. B.P. was supported by a Feodor Lynen Fellowship of the Alexander von Humboldt Foundation. This work was supported by NSF grant no. 91-21263.",

year = "1995",

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doi = "10.1016/0304-3940(95)11803-5",

language = "English (US)",

volume = "195",

pages = "203--205",

journal = "Neuroscience Letters",

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T1 - H2O2 induced lipid peroxidation in rat brain homogenates is greatly reduced by melatonin

AU - Sewerynek, Ewa

AU - Poeggeler, Burkhard

AU - Melchiorri, Daniela

AU - Reiter, Russel J.

N1 - Funding Information: E.S. was supported by a Fogarty International Fellowship, no. 1 FO5 TWO 5013-01 ICP. B.P. was supported by a Feodor Lynen Fellowship of the Alexander von Humboldt Foundation. This work was supported by NSF grant no. 91-21263.

PY - 1995/8/11

Y1 - 1995/8/11

N2 - Melatonin protection against H2O2-induced lipid peroxidation in brain homogenates was measured in vitro. The level of malonaldehyde (MDA) plus 4-hydroxyalkenals (4-HDA) was assayed in brain homogenates as an index of induced membrane oxidative damage. Brain homogenates were co-incubated with H2O2 alone or in combination with melatonin. Brain MDA + 4-HDA increased after H202 (0.1-5 mM) with the effect being both concentration- and time-dependent. Melatonin (0.1-5 mM) protected against H2O2-induced lipid peroxidation of brain homogenates in a concentration-dependent manner.

AB - Melatonin protection against H2O2-induced lipid peroxidation in brain homogenates was measured in vitro. The level of malonaldehyde (MDA) plus 4-hydroxyalkenals (4-HDA) was assayed in brain homogenates as an index of induced membrane oxidative damage. Brain homogenates were co-incubated with H2O2 alone or in combination with melatonin. Brain MDA + 4-HDA increased after H202 (0.1-5 mM) with the effect being both concentration- and time-dependent. Melatonin (0.1-5 mM) protected against H2O2-induced lipid peroxidation of brain homogenates in a concentration-dependent manner.

KW - Brain

KW - HO

KW - Hydroxyl radical

KW - Lipid peroxidation

KW - Melatonin

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JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 3

ER -

H2O2 induced lipid peroxidation in rat brain homogenates is greatly reduced by melatonin

Abstract

Keywords

ASJC Scopus subject areas

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