TY - JOUR
T1 - Guanosine 3',5'-cyclic monophosphate as a mediator of inhibition of renin release
AU - Henrich, W. L.
AU - McAllister, E. A.
AU - Smith, P. B.
AU - Campbell, W. B.
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 1988
Y1 - 1988
N2 - The role of guanosine 3',5'-cyclic monophosphate (cGMP) as an inhibitory mediator of tissue renin release was examined in two different in vitro preparations. In rat superficial cortical slices, renin release stimulated by isoproterenol (10-5 M) was ablated by atriopeptin III (ANP, 2.1 x 10-8 M), nitroprusside (NP, 10-3 M), and 8-bromoguanosine 3',5'-cyclic monophosphate (8-BrcGMP, 10-3 and 10-6 M). Arachidonic acid (10-3 M)-stimulated renin release was also inhibited by ANP and 8-BrcGMP (10-3 and 10-6 M). Both ANP and NP increased tissue cGMP concentrations significantly (P < 0.05), but neither had an effect on adenosine 3',5'-cyclic monophosphate (cAMP) concentrations. When methylene blue (10-5 M), an inhibitor of guanylate cyclase, was added to slices incubated with isoproterenol and ANP, the inhibition of renin release by ANP was abolished. These results were confirmed in a preparation of isolated cultured rat juxtaglomerular cells. In these cells, isoproterenol induced a significant increase (58%, P < 0.01) in renin release, which was inhibited by the addition of 8-BrcGMP (10-6 M). These data demonstrate a direct inhibitory effect of ANP on isoproterenol- and arachidonic acid-induced renin release. The results with NP, 8-BrcGMP, and methylene blue suggest that cGMP is an intracellular mediator of this inhibition.
AB - The role of guanosine 3',5'-cyclic monophosphate (cGMP) as an inhibitory mediator of tissue renin release was examined in two different in vitro preparations. In rat superficial cortical slices, renin release stimulated by isoproterenol (10-5 M) was ablated by atriopeptin III (ANP, 2.1 x 10-8 M), nitroprusside (NP, 10-3 M), and 8-bromoguanosine 3',5'-cyclic monophosphate (8-BrcGMP, 10-3 and 10-6 M). Arachidonic acid (10-3 M)-stimulated renin release was also inhibited by ANP and 8-BrcGMP (10-3 and 10-6 M). Both ANP and NP increased tissue cGMP concentrations significantly (P < 0.05), but neither had an effect on adenosine 3',5'-cyclic monophosphate (cAMP) concentrations. When methylene blue (10-5 M), an inhibitor of guanylate cyclase, was added to slices incubated with isoproterenol and ANP, the inhibition of renin release by ANP was abolished. These results were confirmed in a preparation of isolated cultured rat juxtaglomerular cells. In these cells, isoproterenol induced a significant increase (58%, P < 0.01) in renin release, which was inhibited by the addition of 8-BrcGMP (10-6 M). These data demonstrate a direct inhibitory effect of ANP on isoproterenol- and arachidonic acid-induced renin release. The results with NP, 8-BrcGMP, and methylene blue suggest that cGMP is an intracellular mediator of this inhibition.
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U2 - 10.1152/ajprenal.1988.255.3.f474
DO - 10.1152/ajprenal.1988.255.3.f474
M3 - Article
C2 - 2901230
AN - SCOPUS:0023751848
VL - 255
SP - F474-F478
JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
SN - 0363-6127
IS - 3 (24/3)
ER -