Guanine to adenine(G/A) substitution in the promoter region of the apolipoprotein AI gene and plasma lipid and apolipoprotein profile in Chinese Liangshan Yi and Han

Q. Zhao, P. Shen, H. Zhang

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Objective: Chinese Liangshan Yi, which has very low plasma lipid level and cardiovascular disease morbidity, is important for cardiovascular study. The influence of the guanidine to adenine (G/A) substitution in the promoter region of apolipoprotein AI gene (at 75bp) on plasma lipid and apolipoprotein levels was studied in 691 healthy Chinese Liangshan Yi and Han. Methods: Epidemiological field survey, plasma lipid and apolipoprotein determination, genomic DNA preparation, PCR, PFLP were carried out. Results: (1) Levels of TC, TG, HDL-C, apo AI, and apo B in Yi-farmer sample were significantly lower than those in Yi-emigrant sample (P < 0.01). (2) Yi- emigrants had the similar levels of TC, TG, and apolipoproteins with Liangshan Han people, but its HDL-C level was 7% lower than Han. (3) The relative frequencies of the A allele in the three samples had no evident difference with mean value of 0.23 (Yi-farmer 0.23, Yi-emigrant 0.24, and Han 0.23 respectively), which was similar with that reported in Singapore Chinese (0.275) and much higher than that reported in Ganeasians (P < 0.01). (4) No association of A allele with lipid and apolipoprotein levels was observed in three samples. Conclusion: (1) Yi-farmer has lower plasma lipid and apolipoprotein levels in present time, mainly explained by diet and other environmental factors, and the levels will rise as the dietary cholesterol intake becomes high. (2) Chinese people have evidently higher A allele frequency than Gancasians. Han and other Chinese nationalities may have similar frequencies of A allele. (3) G to A substitution in the promoter-75bp site of apo AI gene has no influence on plasma lipid quantitative phenotypes. This is a first domestic report about the G to A substitution in apo AI gene promoter in Chinese Yi and Han, and the plasma lipid profile in Liangshan Yi.

Original languageEnglish (US)
Pages (from-to)9-12
Number of pages4
JournalChinese Journal of Cardiology
Volume27
Issue number1
StatePublished - 1999

Keywords

  • Chinese Yi
  • Environment
  • Gene
  • Lipids
  • Polymerase chain reaction
  • Polymorphism
  • Restriction fragment length

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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