GTP influences the binding of vincristine in human tumor cytosols

Laura C. Bowman, Janet A. Houghton, Peter J. Houghton

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

The influence of GTP on the formation and stability of [3H]vincristine (VCR)-tubulin complexes in cytosols from two human rhabdomyosarcoma xenografts which have different sensitivities to VCR has been evaluated. After removal of endogenous GTP the initial rate of [3H]VCR binding and the maximal level of bound drug were 2- to 3-fold higher in the presence of 0.1 mM GTP than in its absence. Similarly, the stability of complexes was GTP-dependent. Complex formed from Rh18 tumors, only moderately sensitive to VCR, dissociated at 37° in the absence of GTP with a half-time of 67 min; complex from Rh12 tumors (exquisitely sensitive to VCR) was more stable. Neither complex dissociated in the presence of 0.1 mM GTP over 2 hr examined.

Original languageEnglish (US)
Pages (from-to)695-700
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume135
Issue number3
DOIs
StatePublished - Mar 28 1986
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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