Growth of a water strain of Leptospira in synthetic media

Pertinent parameters of growth of a water strain of Leptospira in synthetic media are described. Growth rates as a function of aeration, temperature, and substrate concentration were determined. Evidence is presented for the production of acetate and CO₂ from long-chain fatty acids. A marked drop in pH was shown to result from the accumulation of acetate during growth in synthetic medium. Growth was initiated at pH values from 5.2 to 7.7. An absolute CO₂ requirement for initiation of growth was demonstrated. Growth is also described with acetate as the sole carbon and energy source, demonstrating that leptospires are capable of fatty acid synthesis.