Transforming growth factor-β (TGFβ) is a multifunctional cytokine showing growth effects on many cell types. In the present study effects of TGFβ1 on mitogen-induced Ca2+ responses were investigated in an immortalized murine mesangial cell line where TGFβ1 effects on growth are inhibitory. TGFβ1 was found to inhibit intracellular Ca2+ mobilization induced by platelet-derived growth factor (PDGF). This effect was completely reversed by previous addition of the non-specific serine/threonine kinase inhibitor H-7, but was unaffected by GF 109203X, a specific inhibitor of protein kinase C (PKC). These findings suggest that inhibition of mitogen-induced Ca2+ mobilization by TGFβ1 appears not to involve prior activation of PKC, but may participate in the mechanisms whereby mesangial cell growth is inhibited by TGFβ1.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jan 1 1995|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology