Group I metal ions of increasing atomic weight suppress the growth of crystalline aggregates and enhance the growth of large, single crystals of T7 RNA polymerase

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Abstract

Crystals of bacteriophage T7 RNA polymerase grown with ammonium phosphate or ammonium sulfate as precipitant and glycerol as a structure stabilizing agent exhibit a strong tendency to grow as aggregates. To identify an agent that would suppress this behavior a large number of metal salts were screened as additives to the crystallization solution. The singly charged group I metal ions (with the exception of lithium) were found to suppress the growth of crystalline aggregates and promote the growth of large, single crystals. The degree to which the growth of aggregates was suppressed and the growth of single crystals was enhanced was clearly and reproducibly correlated with the atomic weight of the metal. Thus, ordered according to their ability to suppress the growth of aggregates and enhance the growth of single crystals, the metal ions were Cs > Rb > K > Na.

Original languageEnglish (US)
Pages (from-to)734-737
Number of pages4
JournalJournal of Crystal Growth
Volume167
Issue number3-4
StatePublished - Oct 1996

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atomic weights
RNA
Metal ions
metal ions
Single crystals
Crystalline materials
single crystals
Metals
ammonium phosphates
bacteriophages
ammonium sulfates
Bacteriophages
Excipients
Ammonium Sulfate
Crystallization
glycerols
Glycerol
Lithium
metals
bacteriophage T7 RNA polymerase

ASJC Scopus subject areas

  • Condensed Matter Physics

Cite this

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abstract = "Crystals of bacteriophage T7 RNA polymerase grown with ammonium phosphate or ammonium sulfate as precipitant and glycerol as a structure stabilizing agent exhibit a strong tendency to grow as aggregates. To identify an agent that would suppress this behavior a large number of metal salts were screened as additives to the crystallization solution. The singly charged group I metal ions (with the exception of lithium) were found to suppress the growth of crystalline aggregates and promote the growth of large, single crystals. The degree to which the growth of aggregates was suppressed and the growth of single crystals was enhanced was clearly and reproducibly correlated with the atomic weight of the metal. Thus, ordered according to their ability to suppress the growth of aggregates and enhance the growth of single crystals, the metal ions were Cs > Rb > K > Na.",
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T1 - Group I metal ions of increasing atomic weight suppress the growth of crystalline aggregates and enhance the growth of large, single crystals of T7 RNA polymerase

AU - Sousa, Rui J

PY - 1996/10

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N2 - Crystals of bacteriophage T7 RNA polymerase grown with ammonium phosphate or ammonium sulfate as precipitant and glycerol as a structure stabilizing agent exhibit a strong tendency to grow as aggregates. To identify an agent that would suppress this behavior a large number of metal salts were screened as additives to the crystallization solution. The singly charged group I metal ions (with the exception of lithium) were found to suppress the growth of crystalline aggregates and promote the growth of large, single crystals. The degree to which the growth of aggregates was suppressed and the growth of single crystals was enhanced was clearly and reproducibly correlated with the atomic weight of the metal. Thus, ordered according to their ability to suppress the growth of aggregates and enhance the growth of single crystals, the metal ions were Cs > Rb > K > Na.

AB - Crystals of bacteriophage T7 RNA polymerase grown with ammonium phosphate or ammonium sulfate as precipitant and glycerol as a structure stabilizing agent exhibit a strong tendency to grow as aggregates. To identify an agent that would suppress this behavior a large number of metal salts were screened as additives to the crystallization solution. The singly charged group I metal ions (with the exception of lithium) were found to suppress the growth of crystalline aggregates and promote the growth of large, single crystals. The degree to which the growth of aggregates was suppressed and the growth of single crystals was enhanced was clearly and reproducibly correlated with the atomic weight of the metal. Thus, ordered according to their ability to suppress the growth of aggregates and enhance the growth of single crystals, the metal ions were Cs > Rb > K > Na.

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