Glycoproteins in membranes of secretory granules of the anterior pituitary gland

Jerzy B. Warchol, Damon C. Herbert, Edward G. Rennels

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Rat anterior pituitary glands were examined by electron microscopy after staining with five different histochemical stains. Histochemical reactions were observed in the cell coat, cell membrane and the membrane surrounding the secretory granules in all anterior pituitary cells following staining with phosphotungstic acid (PTA), chromic acid and PTA, the periodic acid-thiosemicarbazide-silver protein method (PA-TSC-SP) of Thiéry, ruthenium red and concanavalin A. The staining was abolished when the sections were preincubated with pronase, neuraminidase or trypsin and subsequently exposed to PTA, chromic acid and PTA or PA-TSC-SP. The possible functional role of the glycoproteins present in the membrane surrounding the secretory granules is considered.

Original languageEnglish (US)
Pages (from-to)139-146
Number of pages8
JournalHistochemistry
Volume46
Issue number2
DOIs
StatePublished - Feb 1976

ASJC Scopus subject areas

  • Anatomy
  • Agricultural and Biological Sciences(all)

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