Glutathione modulates lipid composition of human colon derived HT-29 cells

M. Madesh, O. Benard, K. A. Balasubramanian

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Glutathione (GSH) is important in maintaining intracellular thiol status. The present study looked at the effect of GSH depletion on lipid composition of colon-derived HT-29 cells. GSH was depleted in HT-29 cells by incubation either with buthionine-S, R-sulfoximine (BSO) or diethylmaleate (DEM). GSH was restored during early periods of cell growth by supplementation of growth medium with either GSH ester or N-acetyl cysteine (NAC). Lipids were analysed following GSH depletion and supplementation. Among the neutral lipids, an increase in free cholesterol and diacylglycerol and decrease in cholesteryl ester and triacylglycerol were seen in GSH-depleted cells as compared to control cells. There were no detectable free fatty acids either in control or GSH-depleted cells. Among the phospholipids, a decrease in phosphatidylcholine and phosphatidylinositol and an increase in phosphatidylethanolamine were observed. These changes were almost completely reversed by supplementation of BSO-treated cells with GSH ester and partially reversed by N-acetyl cysteine. These results suggest that the GSH status of the cell plays an important role in the lipid composition of the cells. Copyright (C) 1998 Elsevier Science Ltd.

Original languageEnglish (US)
Pages (from-to)1345-1352
Number of pages8
JournalInternational Journal of Biochemistry and Cell Biology
Volume30
Issue number12
DOIs
StatePublished - Dec 1 1998
Externally publishedYes

Keywords

  • BSO
  • DEM
  • Glutathione
  • HT-29 cells
  • Lipids
  • N-acetyl cysteine

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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