Glucose transport in human skeletal muscle: The in vivo response to insulin

Riccardo C. Bonadonna, Maria Pia Saccomani, Lynn Seely, Kathleen Starick Zych, Eleuterio Ferrannini, Claudio Cobelli, Ralph A. Defronzo

Research output: Contribution to journalArticlepeer-review

86 Scopus citations

Abstract

Transmembrane glucose transport plays a key role in determining insulin sensitivity. We have measured in vivo WBGU, FGU, and Kin and Kout of 3-O-methyl-D-glucose in forearm skeletal muscle by combining the euglycemic clamp technique, the forearm-balance technique, and a novel dual-tracer (1-[3H]-L-glucose and 3-O-[14C]-methyl-D-glucose) technique for measuring in vivo transmembrane transport. Twenty-seven healthy, lean subjects were studied. During saline infusion, insulin concentration, FGU (n = 6), Kin, and Kout, (n = 4) were similar to baseline. During SRIF-induced hypoinsulinemia (insulin < 15 pM, n = 4) WBGU was close to 0, and FGU, Kin, and Kout were unchanged from basal (insulin = 48 pM) values. During insulin clamps at plasma insulin levels of ∼180 (n = 4), ∼420 (n = 5), ∼3000 (n = 4), and ∼9500 pM (n = 4), WBGU was 14.2 ± 1.3, 34.2 ± 4.1 (P < 0.05 vs. previous step), 55.8 ± 1.8 (P < 0.05 vs. previous step), and 56.1 ± 6.3 μmol · min-1 · kg-1 of body weight (NS vs. previous step), respectively. Graded hyperinsulinemia concomitantly increased FGU from a basal value of 4.7 ± 0.5 μmol · min-1 · kg-1 up to 10.9 ± 2.3 (P < 0.05 vs. basal value), 26.6 ± 4.5 (P < 0.05 vs. previous step), 54.8 ± 4.3 (P < 0.05 vs. previous step), and 61.1 ± 10.8 μmol · min-1 · kg-1 of forearm tissues (NS vs. previous step), respectively. Kin of 3-O-methyl-D-glucose in forearm skeletal muscle was increased by hyperinsulinemia from a basal value of 6.6 · 10-2 ± 0.38 10-2 to 10.0 · 10-2 ± 1.4 · 10-2 (p < 0.05 vs. baseline), 17.2 · 10-2 ± 2.2 · 10-2 (P < 0.05 vs. previous step), 26.3 · 10-2 ± 1.8 · 10-2 (P < 0.05 vs. previous step), and 29.8 · 10-2 ± 5.3 · 10-2 · min-1 (NS vs. previous step), respectively. FGU and Kin were positively correlated (r = 0.88, P < 0.01). Kout of 3-O-methyl-D-glucose did not change from the basal value at the lowest insulin dose (3.9 · 10-2 ± 1.1 · 10-2 vs. 3.8 · 10-2 ± 0.33 · 10-2 · 10-2 · min-1, NS), but rose significantly at the following insulin steps to 6.1 · 10-2 ± 0.8 · 10-2, 6.9 · 10-2 ± 0.5 · 10-2, and 11.9 · 10-2 ± 0.3 · 10-2 · min-1 (P < 0.05 for all three vs. baseline). Thus, in human skeletal muscle, in vivo, insulin stimulates Kin and uptake of glucose in a parallel fashion, whereas SRIF-induced acute hypoinsulinemia does not seem to affect transmembrane transport or uptake of glucose.

Original languageEnglish (US)
Pages (from-to)191-198
Number of pages8
JournalDiabetes
Volume42
Issue number1
StatePublished - Jan 1993
Externally publishedYes

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

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