Glucose responsiveness of a reporter gene transduced into hepatocytic cells using a retroviral vector

Ruihuan Chen, Bruno Doiron, Axel Kahn

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

An MMLV-based retroviral vector containing the chloramphenicol acetyl transferase reporter gene under the control of a glucose-dependent internal promoter derived from the L-type pyruvate kinase gene was constructed. After transfection into psi-CRIP packaging cells, clones producing recombinant retrovirus were selected. These retroviruses were used to infect cultured established hepatocytic cells whose endogenous L-type pyruvate kinase gene is transcriptionally regulated by glucose. In the infected cells, the reporter gene was as responsive to glucose as the endogenous L-type pyruvate kinase gene, and the glucose gene activation was time- and concentration-dependent. The possibility to confer a glucose responsiveness on a transgene carried by a retroviral vector provides a powerful tool in the prospect of gene therapy for diabetes mellitus.

Original languageEnglish (US)
Pages (from-to)223-226
Number of pages4
JournalFEBS Letters
Volume365
Issue number2-3
DOIs
StatePublished - May 29 1995
Externally publishedYes

Keywords

  • Gene therapy
  • Glucose responsiveness
  • Hepatocytic cell
  • Retroviral vector

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Glucose responsiveness of a reporter gene transduced into hepatocytic cells using a retroviral vector'. Together they form a unique fingerprint.

  • Cite this