Glucagon enhances growth of cultured human colorectal cancer cells in vitro

Mary Pat Moyer; J. Bradley Aust; Patricia S. Dixon; Barry A. Levine; Kenneth R. Sirinek

doi:10.1016/0002-9610(85)90406-4

Glucagon enhances growth of cultured human colorectal cancer cells in vitro

Mary Pat Moyer, J. Bradley Aust, Patricia S. Dixon, Barry A. Levine, Kenneth R. Sirinek

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Early passage in vitro cultures of colorectal adenocarcinoma cells were used to determine if glucagon exerts a direct effect on growth of human colon cancer cells. Growth response assays indicated that glucagon generally stimulated growth between 2 and 10 μg/ml, with peak responses at 5 to 10 μg/ml. When glucagon-treated and control cultures were compared, 12 of the 14 cultures (86 percent) were stimulated by glucagon, with an increase in cells from 41 to 100 percent. The other two cultures did not respond to glucagon. These in vitro results suggest that glucagon may enhance growth of most colon cancer cells. Further studies on responsiveness to glucagon may help elucidate mechanisms of oncogenesis and suggest new therapeutic protocols for patients with colorectal cancer.

Original language	English (US)
Pages (from-to)	676-679
Number of pages	4
Journal	The American Journal of Surgery
Volume	150
Issue number	6
DOIs	https://doi.org/10.1016/0002-9610(85)90406-4
State	Published - Dec 1985
Externally published	Yes

ASJC Scopus subject areas

Surgery

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10.1016/0002-9610(85)90406-4

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@article{c88c846734014938b50fbc74f2300d2c,

title = "Glucagon enhances growth of cultured human colorectal cancer cells in vitro",

abstract = "Early passage in vitro cultures of colorectal adenocarcinoma cells were used to determine if glucagon exerts a direct effect on growth of human colon cancer cells. Growth response assays indicated that glucagon generally stimulated growth between 2 and 10 μg/ml, with peak responses at 5 to 10 μg/ml. When glucagon-treated and control cultures were compared, 12 of the 14 cultures (86 percent) were stimulated by glucagon, with an increase in cells from 41 to 100 percent. The other two cultures did not respond to glucagon. These in vitro results suggest that glucagon may enhance growth of most colon cancer cells. Further studies on responsiveness to glucagon may help elucidate mechanisms of oncogenesis and suggest new therapeutic protocols for patients with colorectal cancer.",

author = "Moyer, {Mary Pat} and Aust, {J. Bradley} and Dixon, {Patricia S.} and Levine, {Barry A.} and Sirinek, {Kenneth R.}",

note = "Funding Information: From the Department of Surgery, The University of Texas Health Science Center, San Antonio, Texas. Supported in part by the Morrison Trust, San Antonio, Texas and by Grants R23 CA35933 and ROl CA32722, National Institutes of Health, Bethesda, MD. Requests for reprints should be addressed to Mary Pat Moyer, PhD, Department of Surgery, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, Texas 78284. Presented at the 37th Annual Meeting of the Southwestern Surgical Congress. Las Vegas, Nevada, April 28-May 2. 1985.",

year = "1985",

month = dec,

doi = "10.1016/0002-9610(85)90406-4",

language = "English (US)",

volume = "150",

pages = "676--679",

journal = "The American Journal of Surgery",

issn = "0002-9610",

publisher = "Elsevier Inc.",

number = "6",

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TY - JOUR

T1 - Glucagon enhances growth of cultured human colorectal cancer cells in vitro

AU - Moyer, Mary Pat

AU - Aust, J. Bradley

AU - Dixon, Patricia S.

AU - Levine, Barry A.

AU - Sirinek, Kenneth R.

N1 - Funding Information: From the Department of Surgery, The University of Texas Health Science Center, San Antonio, Texas. Supported in part by the Morrison Trust, San Antonio, Texas and by Grants R23 CA35933 and ROl CA32722, National Institutes of Health, Bethesda, MD. Requests for reprints should be addressed to Mary Pat Moyer, PhD, Department of Surgery, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, Texas 78284. Presented at the 37th Annual Meeting of the Southwestern Surgical Congress. Las Vegas, Nevada, April 28-May 2. 1985.

PY - 1985/12

Y1 - 1985/12

N2 - Early passage in vitro cultures of colorectal adenocarcinoma cells were used to determine if glucagon exerts a direct effect on growth of human colon cancer cells. Growth response assays indicated that glucagon generally stimulated growth between 2 and 10 μg/ml, with peak responses at 5 to 10 μg/ml. When glucagon-treated and control cultures were compared, 12 of the 14 cultures (86 percent) were stimulated by glucagon, with an increase in cells from 41 to 100 percent. The other two cultures did not respond to glucagon. These in vitro results suggest that glucagon may enhance growth of most colon cancer cells. Further studies on responsiveness to glucagon may help elucidate mechanisms of oncogenesis and suggest new therapeutic protocols for patients with colorectal cancer.

AB - Early passage in vitro cultures of colorectal adenocarcinoma cells were used to determine if glucagon exerts a direct effect on growth of human colon cancer cells. Growth response assays indicated that glucagon generally stimulated growth between 2 and 10 μg/ml, with peak responses at 5 to 10 μg/ml. When glucagon-treated and control cultures were compared, 12 of the 14 cultures (86 percent) were stimulated by glucagon, with an increase in cells from 41 to 100 percent. The other two cultures did not respond to glucagon. These in vitro results suggest that glucagon may enhance growth of most colon cancer cells. Further studies on responsiveness to glucagon may help elucidate mechanisms of oncogenesis and suggest new therapeutic protocols for patients with colorectal cancer.

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JO - The American Journal of Surgery

JF - The American Journal of Surgery

IS - 6

ER -

Glucagon enhances growth of cultured human colorectal cancer cells in vitro

Abstract

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