Glomerular endothelial cell proteoglycans - regulation by TGF-β1

Glomerular endothelial cells (G-Endo) may participate in the synthesis of glomerular basement membrane. We studied the metabolism of proteoglycans and its regulation by TGF-β1 in bovine G-Endo. The synthesis of cell layer and medium-associated [³⁵S]SO₄-labeled macromolecules was significantly increased by TGF-β1. On ion exchange chromatography, 80-90% of the ³⁵S-labeled macromolecules in the control medium and cell layer were found to be proteoglycans; TGF-β1 increased their synthesis by 1.8-fold but did not alter the anionic charge density. On dissociative Sepharose CL-4B chromatography, the medium proteoglycans were distributed into two peaks of K_av 0.22 (M-I) and 0.44 (M-II). Digestion procedures showed that 39% and 57% of peaks M-I and M-II consisted of HSPG, the remainder being CS/DSPG. The HSPG in peak M-II has the same hydrodynamic size as HSPG present in bovine glomerular basement membrane (N. Parthasarathy and R. G. Spiro, 1987, J. Biol. Chem. 259:12749). Cell layer proteoglycans also resolved into two peaks of K_av 0.33 (C-I) and 0.63 (C-II), respectively, on Sepharose CL-4B chromatography; 39 and 45% of these peaks were made of HSPG. TGF-β1 increased the synthesis of CS/DSPG in the media by three-fold without affecting the synthesis of HSPG. Our data suggest that G-Endo may participate in synthesis of glomerular matrix HSPG under physiologic conditions; regulation of G-Endo proteoglycans by TGF-β1 may be of relevance in glomerular diseases characterized by matrix expansion.