Global gene expression response to telomerase in bovine adrenocortical cells

Steven D. Perrault, Peter J. Hornsby, Dean H. Betts

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

The infinite proliferative capability of most immortalized cells is dependent upon the presence of the enzyme telomerase and its ability to maintain telomere length and structure. However, telomerase may be involved in a greater system than telomere length regulation, as recent evidence has shown it capable of increasing wound healing in vivo, and improving cellular proliferation rate and survival from apoptosis in vitro. Here, we describe the global gene expression response to ectopic telomerase expression in an in vitro bovine adrenocortical cell model. Telomerase-immortalized cells showed an increased ability for proliferation and survival in minimal essential medium above cells transgenic for GFP. cDNA microarray analyses revealed an altered cell state indicative of increased adrenocortical cell proliferation regulated by the IGF2 pathway and alterations in members of the TGF-B family. As well, we identified alterations in genes associated with development and wound healing that support a model that high telomerase expression induces a highly adaptable, progenitor-like state.

Original languageEnglish (US)
Pages (from-to)925-936
Number of pages12
JournalBiochemical and Biophysical Research Communications
Volume335
Issue number3
DOIs
Publication statusPublished - Sep 30 2005

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Keywords

  • Bovine adrenocortical cells
  • IGF2 pathway
  • Microarray
  • Telomerase
  • Telomere
  • hTERT transgene

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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