@article{2b55786dab474ac58e22a819fa2a702d,
title = "Gli2 mediates the development of castration‑resistant prostate cancer",
abstract = "Glioma-associated oncogene family zinc finger 2 (Gli2), a key component of the hedgehog signaling pathway, has been previously demonstrated to promote the malignant properties of prostate cancer in vitro. However, the role of Gli2 in the development of castration-resistant prostate cancer (CRPC) has yet to be fully elucidated. In the present study, Gli2 expression was knocked down in androgen-responsive prostate cancer cells using an inducible Gli2 short hairpin RNA. Suppression of Gli2 expression resulted in significant reduction of cell viability, increased the proportion of cells in the G0/G1 phases of the cell cycle and reduced the expression of genes associated with cell cycle progression. Gli2 knockdown sensitized both androgen-dependent and -independent prostate cancer cells to the antiandrogen drug Casodex and prevented the outgrowth of LNCaP prostate cancer cells. In addition, Gli2 knockdown significantly suppressed the development of CRPC in a LNCaP xenograft mouse model, which was reversed by the re-expression of Gli2. In conclusion, to the best of our knowledge, the present study was the first occasion in which the essential role of Gli2 in the development of CRPC was demonstrated, providing a potential therapeutic target for the intervention of CRPC.",
keywords = "Castration-resistant prostate cancer, Glioma-associated oncogene family zinc finger 2, Hedgehog signaling, Mouse xenograft, Prostate cancer",
author = "Lu Xia and Hakim Bouamar and Xiang Gu and Carla Zeballos and Tai Qin and Bingzhi Wang and You Zhou and Yuhui Wang and Junhua Yang and Haiyan Zhu and Weishe Zhang and Houghton, {Peter J.} and Sun, {Lu Zhe}",
note = "Funding Information: The present study was in part supported by The Cancer Prevention and Research Institute of Texas (grant no. RP120290-IIRA) and National Institutes of Health (grant no. R01CA172886) to LZS, and National Cancer Institute Cancer Center Support Grant to the Shared Resources of Flow Cytometry and Next Generation Sequencing (grant no. P30 CA054174-17). LX, TQ and BZW were in part supported by a fellowship from Xiangya School of Medicine, Central South University, Changsha, Hunan, P.R. China. XG was in part supported by Cancer Research Training Program Grants from The Cancer Prevention and Research Institute of Texas (grant nos. RP140105 and RP170345). CZ was in part supported by NIH training grants (grant nos. T32CA148724 and F32CA228435). Funding Information: The present study was in part supported by The Cancer Prevention and Research Institute of Texas (grant no. RP120290‑IIRA) and National Institutes of Health (grant no. R01CA172886) to LZS, and National Cancer Institute Cancer Center Support Grant to the Shared Resources of Flow Cytometry and Next Generation Sequencing (grant no. P30 CA054174‑17). LX, TQ and BZW were in part supported by a fellowship from Xiangya School of Medicine, Central South University, Changsha, Hunan, P.R. China. XG was in part supported by Cancer Research Training Program Grants from The Cancer Prevention and Research Institute of Texas (grant nos. RP140105 and RP170345). CZ was in part supported by NIH training grants (grant nos. T32CA148724 and F32CA228435). Publisher Copyright: {\textcopyright} 2020 Spandidos Publications. All rights reserved.",
year = "2020",
month = jul,
doi = "10.3892/ijo.2020.5044",
language = "English (US)",
volume = "57",
pages = "100--112",
journal = "International Journal of Oncology",
issn = "1019-6439",
publisher = "Spandidos Publications",
number = "1",
}