TY - JOUR
T1 - Ginkgo biloba extract EGb 761 has anti-inflammatory properties and ameliorates colitis in mice by driving effector T cell apoptosis
AU - Kotakadi, Venkata S.
AU - Jin, Yu
AU - Hofseth, Anne B.
AU - Ying, Lei
AU - Cui, Xiangli
AU - Volate, Suresh
AU - Chumanevich, Alexander
AU - Wood, Patricia A.
AU - Price, Robert L.
AU - Mcneal, Anna
AU - Singh, Udai P.
AU - Singh, Narendra P.
AU - Nagarkatti, Mitzi
AU - Nagarkatti, Prakash S.
AU - Matesic, Lydia E.
AU - Auclair, Karine
AU - Wargovich, Michael J.
AU - Hofseth, Lorne J.
N1 - Funding Information:
National Institutes of Health (R21DK071541-01 to L.J.H. and M.J.W., 1P01AT003961-01A1 to L.J.H., P.S.N. and M.N. and P20RR17698-01); National Institutes of Health Centres of Biomedical Research Excellence funded University of South Carolina Center for Colon Cancer Research.
Funding Information:
The authors COBRE funded University of South Carolina Center for Colon Cancer Research thank the Statistical Core (Dr Edsel Pena, Director), Pathology Core (Dr William Hrushesky, Director), Administrative Core (Dr Frank Berger, Director), Mouse Core (Dr Marj Pena, Director) and Imaging/ Histology Core supported by the Center for Colon Cancer Research. A special thanks is directed to Mrs Valerie Kennedy for her support in sectioning slides for immunohistochemical staining.
PY - 2008
Y1 - 2008
N2 - Ulcerative colitis is a dynamic, chronic inflammatory condition of the colon associated with an increased colon cancer risk. Ginkgo biloba is a putative antioxidant and has been used for thousands of years to treat a variety of ailments. The aim of this study was to test whether the standardized G.biloba extract, EGb 761, is an antioxidant that can be used to prevent and treat colitis in mice. Here, we show that EGb 761 suppresses the activation of macrophages and can be used to both prevent and treat mouse colitis. Markers of inflammation (iNOS, Cox-2 and tumor necrosis factor-α) and inflammatory stress (p53 and p53-phospho-serine 15) are also downregulated by EGb 761. Furthermore, we show that EGb 761 reduces the numbers of CD4+/CD25-/Foxp3- effector T cells in the colon. Interestingly, EGb 761 drives CD4+ effector T cell apoptosis in vitro and in vivo, providing a mechanistic explanation to the reduction in numbers of this cell type in the colon. This current study is in agreement with previous studies supporting a use of EGb 761 as a complementary and alternative strategy to abate colitis and associated colon cancer.
AB - Ulcerative colitis is a dynamic, chronic inflammatory condition of the colon associated with an increased colon cancer risk. Ginkgo biloba is a putative antioxidant and has been used for thousands of years to treat a variety of ailments. The aim of this study was to test whether the standardized G.biloba extract, EGb 761, is an antioxidant that can be used to prevent and treat colitis in mice. Here, we show that EGb 761 suppresses the activation of macrophages and can be used to both prevent and treat mouse colitis. Markers of inflammation (iNOS, Cox-2 and tumor necrosis factor-α) and inflammatory stress (p53 and p53-phospho-serine 15) are also downregulated by EGb 761. Furthermore, we show that EGb 761 reduces the numbers of CD4+/CD25-/Foxp3- effector T cells in the colon. Interestingly, EGb 761 drives CD4+ effector T cell apoptosis in vitro and in vivo, providing a mechanistic explanation to the reduction in numbers of this cell type in the colon. This current study is in agreement with previous studies supporting a use of EGb 761 as a complementary and alternative strategy to abate colitis and associated colon cancer.
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U2 - 10.1093/carcin/bgn143
DO - 10.1093/carcin/bgn143
M3 - Article
C2 - 18567620
AN - SCOPUS:51849157897
VL - 29
SP - 1799
EP - 1806
JO - Carcinogenesis
JF - Carcinogenesis
SN - 0143-3334
IS - 9
ER -