Gingival, dermal, and periodontal ligament fibroblasts express different extracellular matrix receptors

Archontia A. Palaiologou, Raymond A. Yukna, Randy Moses, Thomas E. Lallier

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

Background: Fibroblasts are the predominant cells of the periodontal ligament and the gingiva and have important roles in the function and regeneration of the tooth support apparatus. The goal of this study was to investigate the possible differences in the adhesion properties and expression of extracellular matrix (ECM) receptors among different fibroblast populations. Methods: The adhesion of gingival (GF), dermal (DF), and periodontal ligament fibroblast (PDLF) cultures to ECM proteins (fibronectin, laminin, vitronectin, RGD peptide, collagen type I, and collagen type IV) adsorbed to tissue culture plastic was evaluated fluorometrically. Quantitative reverse transcription-polymerase chain reactions (RT-PCR) were performed using primers specific for 19 integrin subunits to quantify ECM receptor transcript expression. Results: Our data demonstrated that GF and PDLF adhere to vitronectin and collagen types I and IV more avidly than do DF. PDLF adhered well to laminin, whereas GF and DF did not. Quantitation of integrin expression demonstrated that the different fibroblast types expressed different integrin transcripts, further demonstrating their innate differences. Conclusions: The 3 fibroblast types studied behave differently and expressed different ECM receptors. However, gingival fibroblasts and periodontal ligament fibroblasts are more similar in their attachment and integrin expression than either is to dermal fibroblasts. Therefore, experiments using DF will not necessarily be valid for oral tissues.

Original languageEnglish (US)
Pages (from-to)798-807
Number of pages10
JournalJournal of periodontology
Volume72
Issue number6
DOIs
StatePublished - Jun 1 2001
Externally publishedYes

Keywords

  • Fibroblasts/physiology
  • Periodontal attachment
  • Periodontal ligament
  • Proteins, extracellular matrix

ASJC Scopus subject areas

  • Periodontics

Fingerprint Dive into the research topics of 'Gingival, dermal, and periodontal ligament fibroblasts express different extracellular matrix receptors'. Together they form a unique fingerprint.

  • Cite this