Genotyping of Turkish environmental Cryptococcus neoformans var. neoformans isolates by pulsed field gel electrophoresis and mating type

M. A. Saracli; S. T. Yildiran; K. Sener; A. Gonlum; L. Doganci; S. M. Keller; B. L. Wickes

doi:10.1111/j.1439-0507.2006.01203.x

Genotyping of Turkish environmental Cryptococcus neoformans var. neoformans isolates by pulsed field gel electrophoresis and mating type

M. A. Saracli, S. T. Yildiran, K. Sener, A. Gonlum, L. Doganci, S. M. Keller, B. L. Wickes

Department of Microbiology, Immunology & Molecular Genetics

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

A total of 26 environmental Cryptococcus neoformans var. neoformans strains isolated from 634 samples of pigeon droppings collected from 54 different provinces of Turkey in 1996 and 1997 were included in this study. The results of pulsed-field gel electrophoresis (PFGE) showed that the 26 strains could be separated into 24 different PFGE patterns. In a mating-type study, of 26 strains, 20 were MATα, four were MATa, one was MATa/α and one was non-typable by STE20 specific primers. By the polymerase chain reaction typing, all the isolates were serotype A. The extensive heterogeneity among these isolates suggests that a single clonal population may not be present in Turkey. Additionally, the presence of an AMATa/DMATα hybrid may indicate the existence of strains that are AMATa mating type in Turkish environment.

Original language	English (US)
Pages (from-to)	124-129
Number of pages	6
Journal	Mycoses
Volume	49
Issue number	2
DOIs	https://doi.org/10.1111/j.1439-0507.2006.01203.x
State	Published - Mar 2006

Keywords

Cryptococcus neoformans
Mating type
PFGE
Pulsed-field gel electrophoresis
STE20 primer

ASJC Scopus subject areas

Dermatology
Infectious Diseases

Access to Document

10.1111/j.1439-0507.2006.01203.x

Cite this

@article{2a8f635c4bc94c0ca1539ab79b835774,

title = "Genotyping of Turkish environmental Cryptococcus neoformans var. neoformans isolates by pulsed field gel electrophoresis and mating type",

abstract = "A total of 26 environmental Cryptococcus neoformans var. neoformans strains isolated from 634 samples of pigeon droppings collected from 54 different provinces of Turkey in 1996 and 1997 were included in this study. The results of pulsed-field gel electrophoresis (PFGE) showed that the 26 strains could be separated into 24 different PFGE patterns. In a mating-type study, of 26 strains, 20 were MATα, four were MATa, one was MATa/α and one was non-typable by STE20 specific primers. By the polymerase chain reaction typing, all the isolates were serotype A. The extensive heterogeneity among these isolates suggests that a single clonal population may not be present in Turkey. Additionally, the presence of an AMATa/DMATα hybrid may indicate the existence of strains that are AMATa mating type in Turkish environment.",

keywords = "Cryptococcus neoformans, Mating type, PFGE, Pulsed-field gel electrophoresis, STE20 primer",

author = "Saracli, {M. A.} and Yildiran, {S. T.} and K. Sener and A. Gonlum and L. Doganci and Keller, {S. M.} and Wickes, {B. L.}",

year = "2006",

month = mar,

doi = "10.1111/j.1439-0507.2006.01203.x",

language = "English (US)",

volume = "49",

pages = "124--129",

journal = "Mycoses",

issn = "0933-7407",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "2",

}

TY - JOUR

T1 - Genotyping of Turkish environmental Cryptococcus neoformans var. neoformans isolates by pulsed field gel electrophoresis and mating type

AU - Saracli, M. A.

AU - Yildiran, S. T.

AU - Sener, K.

AU - Gonlum, A.

AU - Doganci, L.

AU - Keller, S. M.

AU - Wickes, B. L.

PY - 2006/3

Y1 - 2006/3

N2 - A total of 26 environmental Cryptococcus neoformans var. neoformans strains isolated from 634 samples of pigeon droppings collected from 54 different provinces of Turkey in 1996 and 1997 were included in this study. The results of pulsed-field gel electrophoresis (PFGE) showed that the 26 strains could be separated into 24 different PFGE patterns. In a mating-type study, of 26 strains, 20 were MATα, four were MATa, one was MATa/α and one was non-typable by STE20 specific primers. By the polymerase chain reaction typing, all the isolates were serotype A. The extensive heterogeneity among these isolates suggests that a single clonal population may not be present in Turkey. Additionally, the presence of an AMATa/DMATα hybrid may indicate the existence of strains that are AMATa mating type in Turkish environment.

AB - A total of 26 environmental Cryptococcus neoformans var. neoformans strains isolated from 634 samples of pigeon droppings collected from 54 different provinces of Turkey in 1996 and 1997 were included in this study. The results of pulsed-field gel electrophoresis (PFGE) showed that the 26 strains could be separated into 24 different PFGE patterns. In a mating-type study, of 26 strains, 20 were MATα, four were MATa, one was MATa/α and one was non-typable by STE20 specific primers. By the polymerase chain reaction typing, all the isolates were serotype A. The extensive heterogeneity among these isolates suggests that a single clonal population may not be present in Turkey. Additionally, the presence of an AMATa/DMATα hybrid may indicate the existence of strains that are AMATa mating type in Turkish environment.

KW - Cryptococcus neoformans

KW - Mating type

KW - PFGE

KW - Pulsed-field gel electrophoresis

KW - STE20 primer

UR - http://www.scopus.com/inward/record.url?scp=33645304878&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645304878&partnerID=8YFLogxK

U2 - 10.1111/j.1439-0507.2006.01203.x

DO - 10.1111/j.1439-0507.2006.01203.x

M3 - Article

C2 - 16466446

AN - SCOPUS:33645304878

SN - 0933-7407

VL - 49

SP - 124

EP - 129

JO - Mycoses

JF - Mycoses

IS - 2

ER -

Genotyping of Turkish environmental Cryptococcus neoformans var. neoformans isolates by pulsed field gel electrophoresis and mating type

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this