Genotyping of Turkish environmental Cryptococcus neoformans var. neoformans isolates by pulsed field gel electrophoresis and mating type

M. A. Saracli, S. T. Yildiran, K. Sener, A. Gonlum, L. Doganci, S. M. Keller, B. L. Wickes

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

A total of 26 environmental Cryptococcus neoformans var. neoformans strains isolated from 634 samples of pigeon droppings collected from 54 different provinces of Turkey in 1996 and 1997 were included in this study. The results of pulsed-field gel electrophoresis (PFGE) showed that the 26 strains could be separated into 24 different PFGE patterns. In a mating-type study, of 26 strains, 20 were MATα, four were MATa, one was MATa/α and one was non-typable by STE20 specific primers. By the polymerase chain reaction typing, all the isolates were serotype A. The extensive heterogeneity among these isolates suggests that a single clonal population may not be present in Turkey. Additionally, the presence of an AMATa/DMATα hybrid may indicate the existence of strains that are AMATa mating type in Turkish environment.

Original languageEnglish (US)
Pages (from-to)124-129
Number of pages6
JournalMycoses
Volume49
Issue number2
DOIs
StatePublished - Mar 2006

Keywords

  • Cryptococcus neoformans
  • Mating type
  • PFGE
  • Pulsed-field gel electrophoresis
  • STE20 primer

ASJC Scopus subject areas

  • Dermatology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Genotyping of Turkish environmental Cryptococcus neoformans var. neoformans isolates by pulsed field gel electrophoresis and mating type'. Together they form a unique fingerprint.

  • Cite this