Genotyping of clinical Rhodotorula mucilaginosa isolates by pulsed field gel electrophoresis

M. A. Saracli; K. Sener; A. Gonlum; S. T. Yildiran; B. L. Wickes

doi:10.1046/j.0933-7407.2003.00925.x

Genotyping of clinical Rhodotorula mucilaginosa isolates by pulsed field gel electrophoresis

M. A. Saracli, K. Sener, A. Gonlum, S. T. Yildiran, B. L. Wickes

Department of Microbiology, Immunology & Molecular Genetics

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Identification and typing of fungal isolates is a prerequisite for control and prevention of nosocomial infections. As the discriminatory power of phenotypic methods is not sufficient for epidemiological purposes, genotyping methods such as DNA fingerprinting, random amplification of polymorphic DNA (RAPD) analysis, or pulsed field gel electrophoresis (PFGE) are preferred. To our knowledge, this study is the first application of PFGE for typing Rhodotorula mucilaginosa strains. The PFGE patterns of six clinical isolates produced two different karyotypes, which were confirmed by RAPD analysis. Five strains isolated from bloodstream infections from three different institutions showed the same karyotype and RAPD patterns, while the urological specimen differed slightly.

Original language	English (US)
Pages (from-to)	487-491
Number of pages	5
Journal	Mycoses
Volume	46
Issue number	11-12
DOIs	https://doi.org/10.1046/j.0933-7407.2003.00925.x
State	Published - 2003

Keywords

Genotyping
Pulsed field gel electrophoresis
Rhodotorula mucilaginosa

ASJC Scopus subject areas

Dermatology
Infectious Diseases

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10.1046/j.0933-7407.2003.00925.x

Cite this

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abstract = "Identification and typing of fungal isolates is a prerequisite for control and prevention of nosocomial infections. As the discriminatory power of phenotypic methods is not sufficient for epidemiological purposes, genotyping methods such as DNA fingerprinting, random amplification of polymorphic DNA (RAPD) analysis, or pulsed field gel electrophoresis (PFGE) are preferred. To our knowledge, this study is the first application of PFGE for typing Rhodotorula mucilaginosa strains. The PFGE patterns of six clinical isolates produced two different karyotypes, which were confirmed by RAPD analysis. Five strains isolated from bloodstream infections from three different institutions showed the same karyotype and RAPD patterns, while the urological specimen differed slightly.",

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AU - Saracli, M. A.

AU - Sener, K.

AU - Gonlum, A.

AU - Yildiran, S. T.

AU - Wickes, B. L.

PY - 2003

Y1 - 2003

N2 - Identification and typing of fungal isolates is a prerequisite for control and prevention of nosocomial infections. As the discriminatory power of phenotypic methods is not sufficient for epidemiological purposes, genotyping methods such as DNA fingerprinting, random amplification of polymorphic DNA (RAPD) analysis, or pulsed field gel electrophoresis (PFGE) are preferred. To our knowledge, this study is the first application of PFGE for typing Rhodotorula mucilaginosa strains. The PFGE patterns of six clinical isolates produced two different karyotypes, which were confirmed by RAPD analysis. Five strains isolated from bloodstream infections from three different institutions showed the same karyotype and RAPD patterns, while the urological specimen differed slightly.

AB - Identification and typing of fungal isolates is a prerequisite for control and prevention of nosocomial infections. As the discriminatory power of phenotypic methods is not sufficient for epidemiological purposes, genotyping methods such as DNA fingerprinting, random amplification of polymorphic DNA (RAPD) analysis, or pulsed field gel electrophoresis (PFGE) are preferred. To our knowledge, this study is the first application of PFGE for typing Rhodotorula mucilaginosa strains. The PFGE patterns of six clinical isolates produced two different karyotypes, which were confirmed by RAPD analysis. Five strains isolated from bloodstream infections from three different institutions showed the same karyotype and RAPD patterns, while the urological specimen differed slightly.

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Genotyping of clinical Rhodotorula mucilaginosa isolates by pulsed field gel electrophoresis

Abstract

Keywords

ASJC Scopus subject areas

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