Genotypic characteristics of HTLV-II isolates from Amerindian and non-Indian populations

Diane Pardi, Brian Hjelle, Thomas M. Folks, Renu B. Lal

    Research output: Contribution to journalArticlepeer-review

    9 Scopus citations

    Abstract

    The Amerindian human T-cell lymphotropic virus type II isolate HTLV-IIG12 has been demonstrated to be an HTLV-IIb with several unique features, including several restriction enzyme site changes, a distinctive pre-gag region, a stop codon within the pol gene, and an extended Tax protein. In this study, HTLV-II isolates from Amerindian and non-Indian populations were characterized by restriction enzyme site analysis to determine the prevalent HTLV-II subtype. In addition, DNA amplification by the polymerase chain reaction and Southern blot analyses were used to probe for the HTLV-IIG12 pre-gag region. Our results showed that of 13 Guaymi Indian isolates subtyped, all were HTLV-IIb, and that approximately one third of 17 isolates had the unique pre-gag region. While other HTLV-II-infected groups contained both HTLV-IIa and HTLV-IIb isolates, none of these isolates showed evidence of the distinctive HTLV-IIG12 pre-gag region. Lastly, DNA sequence analysis was used to determine the prevalence of the stop codon within the pol gene open reading frame. These analyses revealed that the occurrence of a stop codon within this sequence appeared to be characteristic of most HTLV-IIb subtypes. These results further our understanding of the genetic variations and evolution of the HTLV-II viruses within the endemically infected Amerindian populations, as well as U.S. intravenous drug users and other non-Indian populations.

    Original languageEnglish (US)
    Pages (from-to)27-35
    Number of pages9
    JournalVirus Genes
    Volume10
    Issue number1
    DOIs
    StatePublished - Feb 1 1995

    Keywords

    • HTLV-II
    • genotypic variation
    • human T-cell lymphotropic virus type II

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics
    • Virology

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