Genotoxicity of paraquat: Micronuclei induced in bone marrow and peripheral blood are inhibited by melatonin

Genaro G. Ortiz, Russel J. Reiter, Guillermo Zúñiga, Daniela Melchiorri, Ewa Sewerynek, Marta I. Pablos, Chang S. Oh, Joaquín J. García, Oscar K. Bitzer-Quintero

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

The ability of melatonin to influence paraquat-induced genotoxicity was tested using micronucleated polychromatic erythrocytes as an index of damage in both bone marrow and peripheral blood cells of mice. Melatonin (10 mg/kg) or an equal volume of saline were administered intraperitoneally (ip) to mice 30 min prior to an ip injection of paraquat (20 mg/kgx2), and thereafter at 6-h intervals until the conclusion of the study (72 h). The number of the micronucleated polychromatic erythrocytes increased after paraquat administration both in peripheral blood and bone marrow cells. Melatonin administration to paraquat-treated mice significantly reduced micronuclei formation in both peripheral blood and bone marrow cells; these differences were apparent at 24, 48 and 72 h after paraquat administration. The induction of micronuclei was time-dependent with peak values occurring at 24 and 48 h. The reduction in paraquat-related genotoxicity by melatonin is likely due in part to the antioxidant activity of the indole. We did not observe effects of melatonin over paraquat in paraquat+melatonin groups incubated at 0, 60 and 120 min. Mitomycin C, which was used as a positive control, also caused the expected large rises in micronuclei in both bone marrow and peripheral blood cells at 24, 48 and 72 h after its administration. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)239-245
Number of pages7
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume464
Issue number2
DOIs
StatePublished - Jan 24 2000

Keywords

  • Blood
  • Bone marrow
  • Melatonin
  • Micronuclei
  • Paraquat
  • Polychromatic erythrocyte

ASJC Scopus subject areas

  • Genetics
  • Health, Toxicology and Mutagenesis

Fingerprint Dive into the research topics of 'Genotoxicity of paraquat: Micronuclei induced in bone marrow and peripheral blood are inhibited by melatonin'. Together they form a unique fingerprint.

  • Cite this