Genomic transcriptional profiling of the developmental cycle of Chlamydia trachomatis

Robert J. Belland, Guangming Zhong, Deborah D. Crane, Daniel Hogan, Daniel Sturdevant, Jyotika Sharma, Wandy L. Beatty, Harlan D. Caldwell

Research output: Contribution to journalArticle

340 Scopus citations

Abstract

Chlamydia trachomatis is one of the most common bacterial pathogens and is the etiological agent of debilitating sexually transmitted and ocular diseases in humans. The organism is an obligate intracellular prokaryote characterized by a highly specialized biphasic developmental cycle. We have performed genomic transcriptional analysis of the chlamydial developmental cycle. This approach has led to the identification of a small subset of genes that control the primary (immediate-early genes) and secondary (late genes) differentiation stages of the cycle. Immediate-early gene products initiate bacterial metabolism and potentially modify the bacterial phagosome to escape fusion with lysosomes. One immediate early gene (CT147) is a homolog of the human early endosomal antigen-1 that is localized to the chlamydial phagosome; suggesting a functional role for CT147 in establishing the parasitophorous vacuole in a nonfusogenic pathway. Late gene products terminate bacterial cell division and constitute structural components and remodeling activities involved in the formation of the highly disulfide cross-linked outer-membrane complex that functions in attachment and invasion of new host cells. Many of the genes expressed during the immediate-early and late differentiation stages are Chlamydia-specific and have evolutionary origins in eukaryotic lineages.

Original languageEnglish (US)
Pages (from-to)8478-8483
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number14
DOIs
StatePublished - Jul 8 2003

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Genomic transcriptional profiling of the developmental cycle of Chlamydia trachomatis'. Together they form a unique fingerprint.

  • Cite this