Genomic organization of the simian virus 40-adenovirus 7 hybrid virus, PARA(cT), that encodes a nuclear transport defective simian virus 40 T antigen

Robert E. Lanford, James R. Jacob, Janet S. Butel

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    Abstract

    The genomic organization of the simian virus 40 (SV40)-adenovirus (Ad)7 hybrid virus, PARA(cT), was examined. A deletion of approximately 5529 by of Ad7 DNA extends from 78.8 map units to 94.0 map units and is replaced by an SV40 DNA insert of 3809 bp. The left-hand end of the insertion begins at SV40 nucleotide 5168, 5 by upstream of the ATG initiation codon for T-ag synthesis. The sequence extends counterclockwise through the T-ag encoding sequences and into SV40 late region DNA. Most of the late region DNA has been removed in a deletion between nucleotides 2464 and 301. One of the 72-bp repeats has also been deleted. The right-hand end of the SV40 DNA insert is at nucleotide 4366. Thus, a portion of the SV40 DNA early region is repeated at both ends of the insert (nucleotides 5168-4366).

    Original languageEnglish (US)
    Pages (from-to)271-276
    Number of pages6
    JournalVirology
    Volume155
    Issue number1
    DOIs
    StatePublished - Nov 1986

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    ASJC Scopus subject areas

    • Virology

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