Genomic organization of the simian virus 40-adenovirus 7 hybrid virus, PARA(cT), that encodes a nuclear transport defective simian virus 40 T antigen

Robert E. Lanford, James R. Jacob, Janet S. Butel

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The genomic organization of the simian virus 40 (SV40)-adenovirus (Ad)7 hybrid virus, PARA(cT), was examined. A deletion of approximately 5529 by of Ad7 DNA extends from 78.8 map units to 94.0 map units and is replaced by an SV40 DNA insert of 3809 bp. The left-hand end of the insertion begins at SV40 nucleotide 5168, 5 by upstream of the ATG initiation codon for T-ag synthesis. The sequence extends counterclockwise through the T-ag encoding sequences and into SV40 late region DNA. Most of the late region DNA has been removed in a deletion between nucleotides 2464 and 301. One of the 72-bp repeats has also been deleted. The right-hand end of the SV40 DNA insert is at nucleotide 4366. Thus, a portion of the SV40 DNA early region is repeated at both ends of the insert (nucleotides 5168-4366).

Original languageEnglish (US)
Pages (from-to)271-276
Number of pages6
JournalVirology
Volume155
Issue number1
DOIs
StatePublished - 1986
Externally publishedYes

Fingerprint

Defective Viruses
Simian virus 40
Cell Nucleus Active Transport
Viral Tumor Antigens
Adenoviridae
Viruses
DNA
Nucleotides
Hand
Initiator Codon

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Genomic organization of the simian virus 40-adenovirus 7 hybrid virus, PARA(cT), that encodes a nuclear transport defective simian virus 40 T antigen. / Lanford, Robert E.; Jacob, James R.; Butel, Janet S.

In: Virology, Vol. 155, No. 1, 1986, p. 271-276.

Research output: Contribution to journalArticle

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