Genomic organization and localization of mouse NMA/BAMBI: Possible implications related to ameloblastoma formation

C. Knight, P. Papagerakis, D. Simmons, A. Berdal, M. MacDougall

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Our laboratory has determined the DNA sequence and transcriptional expression pattern of a mouse cDNA clone termed Nma/BAMBI. This clone encodes a highly conserved protein with 89% identity to the human homologue (termed Nma) and 78% similarity to the Xenopus homologue (termed BAMBI) at the predicted amino acid level. Nma/BAMBI encodes a 260-amino-acid transmembrane protein that has homology to the transforming growth factor (TGF) β type I receptor family. This protein contains an extracellular ligand binding domain, a 24-amino-acid transmembrane domain, and a short intracellular domain that lacks a functional serine/threonine kinase domain. It is believed that Nma/BAMBI is important in the negative regulation of TGFβ signal transduction pathways during development and has implications in tumor progression. We have determined the genomic organization of the mouse Nma/BAMBI gene and confirmed the chromosomal mapping to human chromosome 10 and mouse chromosome 2. Furthermore, we report the production and utilization of an anti-peptide antibody in preliminary immunohistochemical analysis of an ameloblastoma.

Original languageEnglish (US)
Pages (from-to)359-364
Number of pages6
JournalConnective Tissue Research
Volume43
Issue number2-3
Publication statusPublished - Jul 27 2002

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Keywords

  • Ameloblastoma
  • Bone morphogenic protein and activin membrane bound inhibitor (BAMBI)
  • Chromosome 10
  • Nonmetastatic gene A (Nma)
  • TGFβ type I receptor

ASJC Scopus subject areas

  • Rheumatology
  • Biochemistry
  • Orthopedics and Sports Medicine
  • Molecular Biology
  • Cell Biology

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