Genomic heterogeneity of AIDS retroviral isolates from North America and Zaire

Steven Benn, Rosamond Rutledge, Thomas Folks, Jonathan Gold, Louis Baker, Joseph McCormick, Paul Feorino, Peter Piot, Thomas Quinn, Malcolm Martin

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    Abstract

    In an analysis of the genomic variation of AIDS retroviral isolates from patients living in New York, Alabama, and Zaire, restriction maps were constructed by using seven enzymes, each known to cleave the proviral DNA more than once, in conjunction with Southern blot analysis. The maps of LAV, HTLV-III, and ARV-2 as deduced from their published nucleotide sequences were included in this analysis. The results demonstrated that (i) several "signature" restriction sites were common to all isolates; (ii) with the exception of LAV and HTLV-III, the North American and European isolates were all different from one another and showed no geographical specificity; (iii) the African isolates as a group were more diverse than those from North America and Europe; and (iv) the genomic variability was concentrated within the env gene.

    Original languageEnglish (US)
    Pages (from-to)949-951
    Number of pages3
    JournalScience
    Volume230
    Issue number4728
    DOIs
    Publication statusPublished - Jan 1 1985

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    ASJC Scopus subject areas

    • General

    Cite this

    Benn, S., Rutledge, R., Folks, T., Gold, J., Baker, L., McCormick, J., ... Martin, M. (1985). Genomic heterogeneity of AIDS retroviral isolates from North America and Zaire. Science, 230(4728), 949-951. https://doi.org/10.1126/science.2997922