Genome-wide methylation profiling in archival formalin-fixed paraffin-embedded tissue samples

J. Keith Killian, Robert L. Walker, Sven Bilke, Yidong Chen, Sean Davis, Robert Cornelison, William I. Smith, Paul S. Meltzer

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

New technologies allow for genome-scale measurement of DNA methylation. In an effort to increase the clinical utility of DNA methylation as a biomarker, we have adapted a commercial bisulfite epigenotyping assay for genome-wide methylation profiling in archival formalin-fixed paraffin-embedded pathology specimens. This chapter takes the reader step by step through a biomarker discovery experiment to identify phenotype-correlated DNA methylation signatures in routine pathology specimens.

Original languageEnglish (US)
Title of host publicationMolecular Profiling
Subtitle of host publicationMethods and Protocols
EditorsVirginia Espina, Lance Liotta
Pages107-118
Number of pages12
DOIs
StatePublished - 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume823
ISSN (Print)1064-3745

Keywords

  • Biomarker
  • DNA methylation
  • Epigenetics
  • FFPE
  • Pathology

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Killian, J. K., Walker, R. L., Bilke, S., Chen, Y., Davis, S., Cornelison, R., Smith, W. I., & Meltzer, P. S. (2012). Genome-wide methylation profiling in archival formalin-fixed paraffin-embedded tissue samples. In V. Espina, & L. Liotta (Eds.), Molecular Profiling: Methods and Protocols (pp. 107-118). (Methods in Molecular Biology; Vol. 823). https://doi.org/10.1007/978-1-60327-216-2_8