Abstract
Elucidating the genetic architecture of preeclampsia is a major goal in obstetric medicine. We have performed a genome-wide association study (GWAS) for preeclampsia in unrelated Australian individuals of Caucasian ancestry using the Illumina OmniExpress-12 BeadChip to successfully genotype 648,175 SNPs in 538 preeclampsia cases and 540 normal pregnancy controls. Two SNP associations (rs7579169, p = 3.58×10 -7, OR = 1.57; rs12711941, p = 4.26×10 -7, OR = 1.56) satisfied our genome-wide significance threshold (modified Bonferroni p<5.11×10 -7). These SNPs reside in an intergenic region less than 15 kb downstream from the 3′ terminus of the Inhibin, beta B (INHBB) gene on 2q14.2. They are in linkage disequilibrium (LD) with each other (r 2 = 0.92), but not (r 2<0.80) with any other genotyped SNP ±250 kb. DNA re-sequencing in and around the INHBB structural gene identified an additional 25 variants. Of the 21 variants that we successfully genotyped back in the case-control cohort the most significant association observed was for a third intergenic SNP (rs7576192, p = 1.48×10 -7, OR = 1.59) in strong LD with the two significant GWAS SNPs (r 2>0.92). We attempted to provide evidence of a putative regulatory role for these SNPs using bioinformatic analyses and found that they all reside within regions of low sequence conservation and/or low complexity, suggesting functional importance is low. We also explored the mRNA expression in decidua of genes ±500 kb of INHBB and found a nominally significant correlation between a transcript encoded by the EPB41L5 gene, ~250 kb centromeric to INHBB, and preeclampsia (p = 0.03). We were unable to replicate the associations shown by the significant GWAS SNPs in case-control cohorts from Norway and Finland, leading us to conclude that it is more likely that these SNPs are in LD with as yet unidentified causal variant(s).
| Original language | English (US) |
|---|---|
| Article number | e33666 |
| Journal | PLoS One |
| Volume | 7 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 14 2012 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
Cite this
Genome-wide association scan identifies a risk locus for preeclampsia on 2q14, near the inhibin, beta B gene. / Johnson, Matthew P.; Brennecke, Shaun P.; East, Christine E.; Göring, Harald H H; Kent, Jack W.; Dyer, Thomas D.; Said, Joanne M.; Roten, Linda T.; Iversen, Ann Charlotte; Abraham, Lawrence J.; Heinonen, Seppo; Kajantie, Eero; Kere, Juha; Kivinen, Katja; Pouta, Anneli; Laivuori, Hannele; Austgulen, Rigmor; Blangero, John; Moses, Eric K.
In: PLoS One, Vol. 7, No. 3, e33666, 14.03.2012.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genome-wide association scan identifies a risk locus for preeclampsia on 2q14, near the inhibin, beta B gene
AU - Johnson, Matthew P.
AU - Brennecke, Shaun P.
AU - East, Christine E.
AU - Göring, Harald H H
AU - Kent, Jack W.
AU - Dyer, Thomas D.
AU - Said, Joanne M.
AU - Roten, Linda T.
AU - Iversen, Ann Charlotte
AU - Abraham, Lawrence J.
AU - Heinonen, Seppo
AU - Kajantie, Eero
AU - Kere, Juha
AU - Kivinen, Katja
AU - Pouta, Anneli
AU - Laivuori, Hannele
AU - Austgulen, Rigmor
AU - Blangero, John
AU - Moses, Eric K.
PY - 2012/3/14
Y1 - 2012/3/14
N2 - Elucidating the genetic architecture of preeclampsia is a major goal in obstetric medicine. We have performed a genome-wide association study (GWAS) for preeclampsia in unrelated Australian individuals of Caucasian ancestry using the Illumina OmniExpress-12 BeadChip to successfully genotype 648,175 SNPs in 538 preeclampsia cases and 540 normal pregnancy controls. Two SNP associations (rs7579169, p = 3.58×10 -7, OR = 1.57; rs12711941, p = 4.26×10 -7, OR = 1.56) satisfied our genome-wide significance threshold (modified Bonferroni p<5.11×10 -7). These SNPs reside in an intergenic region less than 15 kb downstream from the 3′ terminus of the Inhibin, beta B (INHBB) gene on 2q14.2. They are in linkage disequilibrium (LD) with each other (r 2 = 0.92), but not (r 2<0.80) with any other genotyped SNP ±250 kb. DNA re-sequencing in and around the INHBB structural gene identified an additional 25 variants. Of the 21 variants that we successfully genotyped back in the case-control cohort the most significant association observed was for a third intergenic SNP (rs7576192, p = 1.48×10 -7, OR = 1.59) in strong LD with the two significant GWAS SNPs (r 2>0.92). We attempted to provide evidence of a putative regulatory role for these SNPs using bioinformatic analyses and found that they all reside within regions of low sequence conservation and/or low complexity, suggesting functional importance is low. We also explored the mRNA expression in decidua of genes ±500 kb of INHBB and found a nominally significant correlation between a transcript encoded by the EPB41L5 gene, ~250 kb centromeric to INHBB, and preeclampsia (p = 0.03). We were unable to replicate the associations shown by the significant GWAS SNPs in case-control cohorts from Norway and Finland, leading us to conclude that it is more likely that these SNPs are in LD with as yet unidentified causal variant(s).
AB - Elucidating the genetic architecture of preeclampsia is a major goal in obstetric medicine. We have performed a genome-wide association study (GWAS) for preeclampsia in unrelated Australian individuals of Caucasian ancestry using the Illumina OmniExpress-12 BeadChip to successfully genotype 648,175 SNPs in 538 preeclampsia cases and 540 normal pregnancy controls. Two SNP associations (rs7579169, p = 3.58×10 -7, OR = 1.57; rs12711941, p = 4.26×10 -7, OR = 1.56) satisfied our genome-wide significance threshold (modified Bonferroni p<5.11×10 -7). These SNPs reside in an intergenic region less than 15 kb downstream from the 3′ terminus of the Inhibin, beta B (INHBB) gene on 2q14.2. They are in linkage disequilibrium (LD) with each other (r 2 = 0.92), but not (r 2<0.80) with any other genotyped SNP ±250 kb. DNA re-sequencing in and around the INHBB structural gene identified an additional 25 variants. Of the 21 variants that we successfully genotyped back in the case-control cohort the most significant association observed was for a third intergenic SNP (rs7576192, p = 1.48×10 -7, OR = 1.59) in strong LD with the two significant GWAS SNPs (r 2>0.92). We attempted to provide evidence of a putative regulatory role for these SNPs using bioinformatic analyses and found that they all reside within regions of low sequence conservation and/or low complexity, suggesting functional importance is low. We also explored the mRNA expression in decidua of genes ±500 kb of INHBB and found a nominally significant correlation between a transcript encoded by the EPB41L5 gene, ~250 kb centromeric to INHBB, and preeclampsia (p = 0.03). We were unable to replicate the associations shown by the significant GWAS SNPs in case-control cohorts from Norway and Finland, leading us to conclude that it is more likely that these SNPs are in LD with as yet unidentified causal variant(s).
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UR - http://www.scopus.com/inward/citedby.url?scp=84863399988&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0033666
DO - 10.1371/journal.pone.0033666
M3 - Article
C2 - 22432041
AN - SCOPUS:84863399988
VL - 7
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 3
M1 - e33666
ER -