Genome-wide analysis of alternative promoters of human genes using a custom promoter tiling array

Gregory A.C. Singer, Jiejun Wu, Pearlly Yan, Christoph Plass, Tim H.M. Huang, Ramana V. Davuluri

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Background: Independent lines of evidence suggested that a large fraction of human genes possess multiple promoters driving gene expression from distinct transcription start sites. Understanding which promoter is employed in which cellular context is required to unravel gene regulatory networks within the cell. Results: We have developed a custom microarray platform that tiles roughly 35,000 alternative putative promoters from nearly 7,000 genes in the human genome. To demonstrate the utility of this array platform, we have analyzed the patterns of promoter usage in 17β-estradiol (E2)-treated and untreated MCF7 cells and show widespread usage of alternative promoters. Most intriguingly, we show that the downstream promoter in E2-sensitive multiple promoter genes tends to be very close to the 3'-terminus of the gene, suggesting exotic mechanisms of expression regulation in these genes. Conclusion: The usage of alternative promoters greatly multiplies the transcriptional complexity available within the human genome. The fact that many of these promoters are incapable of driving the synthesis of a meaningful protein-encoding transcript further complicates the story.

Original languageEnglish (US)
Article number349
JournalBMC genomics
Volume9
DOIs
StatePublished - Jul 25 2008
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

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