Genistein alters methylation patterns in mice

J. Kevin Day, Andrew M. Bauer, Charles Desbordes, Yi Zhuang, Byung Eun Kim, Leslie G. Newton, Vedika Nehra, Kara M. Forsee, Ruth S. MacDonald, Cynthia Besch-Williford, Hui-ming Huang, Dennis B. Lubahn

Research output: Contribution to journalArticle

164 Citations (Scopus)

Abstract

In this study we examine the effect of the phytoestrogen genistein on DNA methylation. DNA methylation is thought to inhibit transcription of genes by regulating alterations in chromatin structure. Estrogenic compounds have been reported to regulate DNA methylation in a small number of studies. Additionally, phytoestrogens are believed to affect progression of some human diseases, such as estrogen-dependent cancers, osteoporosis and cardiovascular disease. Specifically, our working hypothesis is that certain soy phytoestrogens, such as genistein, may be involved in preventing the development of certain prostate and mammary cancers by maintaining a protective DNA methylation profile. The objective of the present study is to use mouse differential methylation hybridization (DMH) arrays to test for changes in the methylation status of the cytosine guanine dinucleotide (CpG) islands in the mouse genome by examining how these methylation patterns are affected by genistein. Male mice were fed a casein-based diet (control) or the same diet containing 300 mg genistein/kg according to one of four regimens: control diet for 4 wk, genistein diet for 4 wk, control diet for 2 wk followed by genistein diet for 2 wk and genistein diet for 2 wk followed by control diet for 2 wk. DNA from liver, brain and prostate were then screened with DMH arrays. Clones with methylation differences were sequenced and compared with known sequences. In conclusion, consumption of genistein diet was positively correlated with changes in prostate DNA methylation at CpG islands of specific mouse genes.

Original languageEnglish (US)
JournalJournal of Nutrition
Volume132
Issue number8 SUPPL.
StatePublished - 2002
Externally publishedYes

Fingerprint

Genistein
genistein
methylation
Methylation
Diet
DNA methylation
DNA Methylation
mice
diet
Phytoestrogens
plant estrogens
CpG Islands
Prostate
Estrogens
hybridization
neoplasms
cytosine
guanine
osteoporosis
Caseins

Keywords

  • DNA methylation
  • Genistein
  • Phytoestrogen

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Kevin Day, J., Bauer, A. M., Desbordes, C., Zhuang, Y., Kim, B. E., Newton, L. G., ... Lubahn, D. B. (2002). Genistein alters methylation patterns in mice. Journal of Nutrition, 132(8 SUPPL.).

Genistein alters methylation patterns in mice. / Kevin Day, J.; Bauer, Andrew M.; Desbordes, Charles; Zhuang, Yi; Kim, Byung Eun; Newton, Leslie G.; Nehra, Vedika; Forsee, Kara M.; MacDonald, Ruth S.; Besch-Williford, Cynthia; Huang, Hui-ming; Lubahn, Dennis B.

In: Journal of Nutrition, Vol. 132, No. 8 SUPPL., 2002.

Research output: Contribution to journalArticle

Kevin Day, J, Bauer, AM, Desbordes, C, Zhuang, Y, Kim, BE, Newton, LG, Nehra, V, Forsee, KM, MacDonald, RS, Besch-Williford, C, Huang, H & Lubahn, DB 2002, 'Genistein alters methylation patterns in mice', Journal of Nutrition, vol. 132, no. 8 SUPPL..
Kevin Day J, Bauer AM, Desbordes C, Zhuang Y, Kim BE, Newton LG et al. Genistein alters methylation patterns in mice. Journal of Nutrition. 2002;132(8 SUPPL.).
Kevin Day, J. ; Bauer, Andrew M. ; Desbordes, Charles ; Zhuang, Yi ; Kim, Byung Eun ; Newton, Leslie G. ; Nehra, Vedika ; Forsee, Kara M. ; MacDonald, Ruth S. ; Besch-Williford, Cynthia ; Huang, Hui-ming ; Lubahn, Dennis B. / Genistein alters methylation patterns in mice. In: Journal of Nutrition. 2002 ; Vol. 132, No. 8 SUPPL.
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