Genetic variation in PARL influences mitochondrial content

Joanne E. Curran, Jeremy B.M. Jowett, Lawrence J. Abraham, Luke A. Diepeveen, Katherine S. Elliott, Thomas D. Dyer, Lyndal J. Kerr-Bayles, Matthew P. Johnson, Anthony G. Comuzzie, Eric K. Moses, Ken R. Walder, Gregory R. Collier, John Blangero, Ahmed H. Kissebah

    Research output: Contribution to journalArticle

    15 Scopus citations

    Abstract

    Given their involvement in processes necessary for life, mitochondrial damage and subsequent dysfunction can lead to a wide range of human diseases. Previous studies of both animal models and humans have suggested that presenilins-associated rhomboid-like protein (PARL) is a key regulator of mitochondrial integrity and function, and plays a role in cellular apoptosis. As a surrogate measure of mitochondrial integrity, we previously measured mitochondrial content in a Caucasian population consisting of large extended pedigrees, with results highlighting a substantial genetic component to this trait. To assess the influence of variation in the PARL gene on mitochondrial content, we re-sequenced 6.5 kb of the gene, identifying 16 SNPs and genotyped these in 1,086 Caucasian individuals, distributed across 170 families. Statistical genetic analysis revealed that one promoter variant, T-191C, exhibited significant effects (after correction for multiple testing) on mitochondrial content levels. Comparison of the transcription factor binding characteristics of the T-191C promoter SNP by EMSA indicates preferential binding of nuclear factors to the T allele, suggesting functional variation in PARL expression. These results suggest that genetic variation within PARL influences mitochondrial abundance and integrity.

    Original languageEnglish (US)
    Pages (from-to)183-190
    Number of pages8
    JournalHuman Genetics
    Volume127
    Issue number2
    DOIs
    StatePublished - 2010

    ASJC Scopus subject areas

    • Genetics
    • Genetics(clinical)

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  • Cite this

    Curran, J. E., Jowett, J. B. M., Abraham, L. J., Diepeveen, L. A., Elliott, K. S., Dyer, T. D., Kerr-Bayles, L. J., Johnson, M. P., Comuzzie, A. G., Moses, E. K., Walder, K. R., Collier, G. R., Blangero, J., & Kissebah, A. H. (2010). Genetic variation in PARL influences mitochondrial content. Human Genetics, 127(2), 183-190. https://doi.org/10.1007/s00439-009-0756-0