Genetic Mapping of Vascular Calcified Plaque Loci on Chromosome 16p in European Americans from the Diabetes Heart Study

Allison B. Lehtinen, Amanda J. Cox, Julie T. Ziegler, V. Saroja Voruganti, Jianzhao Xu, Barry I. Freedman, J. Jeffrey Carr, Anthony G. Comuzzie, Carl D. Langefeld, Donald W. Bowden

    Research output: Contribution to journalArticlepeer-review

    5 Scopus citations

    Abstract

    A linkage peak for carotid artery calcified plaque (CarCP) on chromosome 16p (LOD 4.39 at 8.4 cM) in families with type 2 diabetes mellitus (T2DM) from the Diabetes Heart Study (DHS) has been refined. Fine mapping encompassed 104 single-nucleotide polymorphisms (SNPs) in 937 subjects from 315 families; including 45 SNPs in six candidate genes (CACNA1H, SEPX1, ABCA3, IL32, SOCS1, CLEC16A). Linkage and association analyses using variance components analysis adjusting for age, gender, body mass index (BMI), and diabetes status refined the CarCP linkage into two distinct peaks (LODs: 3.89 at 6.9 cM and 4.86 at 16.0 cM). Evidence of linkage for coronary calcified plaque (LOD: 2.27 at 19 cM) and a vascular calcification principle component (LOD: 3.71 at 16.0 cM) was also observed. The strongest evidence for association with CarCP was observed with SNPs in the A2BP1 gene region (rs4337300 P= 0.005) with modest evidence of association with SNPs in CACNA1H (P= 0.010-0.033). Bayesian quantitative trait nucleotide (BQTN) analysis identified a SNP, rs1358489, with either a functional effect on CarCP or in linkage disequilibrium (LD) with a functional SNP. This study refined the 16p region contributing to vascular calcification. The causal variants remain to be identified, but results are consistent with a linkage peak that is due to multiple common variants, though rare variants cannot be excluded.

    Original languageEnglish (US)
    Pages (from-to)222-235
    Number of pages14
    JournalAnnals of Human Genetics
    Volume75
    Issue number2
    DOIs
    StatePublished - Mar 2011

    Keywords

    • Fine mapping
    • Subclinical cardiovascular disease
    • Type 2 diabetes

    ASJC Scopus subject areas

    • Genetics
    • Genetics(clinical)

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