Genetic manipulation of the mouse via gene targeting in embryonic stem cells.

A. Bradley, R. Ramírez-Solis, H. Zheng, P. Hasty, A. Davis

Research output: Contribution to journalReview article

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Abstract

Gene targeting applied to totipotent embryonic stem (ES) cells is a very powerful means of creating highly specific mutations of genes in the mouse. The successful application of this technology is however constrained by both the types of mutations that can be generated at a target locus and the ability to reconstruct a germline chimera from the manipulated cells. We have developed two cell lines that can be routinely transmitted through the germline of chimeras after cloning and prolonged selection in tissue culture. We have also established a variety of methods for generating non-selected mutations at the X-linked hprt locus in ES cells. Our observations at this locus have enabled us to generate successfully a subtle mutation at the non-selectable Hox-2.6 locus.

Original languageEnglish (US)
Pages (from-to)256-269; discussion 269-276
JournalCiba Foundation symposium
Volume165
StatePublished - 1992
Externally publishedYes

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    Bradley, A., Ramírez-Solis, R., Zheng, H., Hasty, P., & Davis, A. (1992). Genetic manipulation of the mouse via gene targeting in embryonic stem cells. Ciba Foundation symposium, 165, 256-269; discussion 269-276.