Genetic analysis of praziquantel response in schistosome parasites implicates a transient receptor potential channel

Winka Le Clec'h, Frédéric D. Chevalier, Ana Carolina A. Mattos, Amanda Strickland, Robbie Diaz, Marina McDew-White, Claudia M. Rohr, Safari Kinung'hi, Fiona Allan, Bonnie L. Webster, Joanne P. Webster, Aidan M. Emery, David Rollinson, Amadou Garba Djirmay, Khalid M. Al Mashikhi, Salem Al Yafae, Mohamed A. Idris, Hélène Moné, Gabriel Mouahid, Philip LoVerdeJonathan S. Marchant, Timothy J.C. Anderson

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


Mass drug administration with praziquantel (PZQ) monotherapy is considered the mainstay for control and elimination of the parasites causing schistosomiasis in humans. This drug shows imperfect cure rates in the field, and parasites showing reduced PZQ response can be selected in the laboratory, but the extent of resistance in Schistosoma mansoni populations is unknown. We examined the genetic basis of the variation in response in a PZQ-selected S. mansoni population (SmLE-PZQ-R) in which 35% of the parasitic worms survive high-dose PZQ (73 micrograms per milliliter) treatment. We used genome-wide association to map loci underlying PZQ response and identified a transient receptor potential (Sm.TRPMPZQ) channel (Smp_246790) within the major chromosome 3 peak that is activated by nanomolar concentrations of PZQ. The PZQ response showed recessive inheritance and marker-assisted selection of parasites at a single Sm.TRPMPZQ SNP that produced populations of PZQ-enriched resistant (PZQ-ER) and PZQ-enriched sensitive (PZQ-ES) parasites, exhibiting >377-fold difference in PZQ response. The PZQ-ER parasites survived treatment in rodents at higher frequencies compared with PZQ-ES, and resistant parasites exhibited 2.25-fold lower expression of Sm.TRPMPZQ relative to sensitive parasites. Specific chemical blockers of Sm.TRPMPZQ enhanced PZQ resistance, whereas Sm.TRPMPZQ activators increased sensitivity. We surveyed Sm.TRPMPZQ sequence variations in 259 parasites from different global sites and identified one nonsense mutation that resulted in a truncated protein with no PZQ binding site. Our results demonstrate that Sm.TRPMPZQ underlies variation in PZQ responses in S. mansoni and provides an approach for monitoring emerging PZQ-resistant alleles in schistosome elimination programs.

Original languageEnglish (US)
Article numberabj9114
JournalScience translational medicine
Issue number625
StatePublished - Dec 22 2021
Externally publishedYes

ASJC Scopus subject areas

  • General Medicine


Dive into the research topics of 'Genetic analysis of praziquantel response in schistosome parasites implicates a transient receptor potential channel'. Together they form a unique fingerprint.

Cite this